Construction of a lncRNA-associated competing endogenous RNA regulatory network after traumatic brain injury in mouse

© 2022. The Author(s)..

Traumatic brain injury (TBI) is a major public health problem worldwide which causes high mortality and disability. Functioning as microRNA (miRNA) sponges, long non-coding RNA (lncRNA) regulates the expression of protein-coding genes in a competing endogenous RNA (ceRNA) network. However, the lncRNA-associated ceRNA in TBI remains unclear. In this study, we processed the raw SRR files of mice cortex samples of sham injury (n = 3) and TBI groups (n = 3) to count files. Then, the expression profiles of lncRNAs and mRNAs were identified, and 86 differentially expressed (DE) lncRNAs and 1201 DEmRNAs between sham and TBI groups were identified. The DEmRNAs were used to perform enrichment analyses. Next, a lncRNA-miRNA-mRNA regulatory ceRNA network was constructed. The network consisted of 23 mRNAs, 5 miRNAs and 2 lncRNAs. The expression alternations of the 5 miRNAs were validated via qRT-PCR. The subnetwork of hub lncRNA Neat1 was extracted. We identified a potential inflammatory associated regulatory axis: Neat1/miR-31-5p/Myd88 axis. The PPI network based on DEmRNA involved in ceRNA network was constructed PPI networks to identify the hub genes. Finally, DElncRNAs and DEmRNAs were selected randomly and validated by qRT-PCR. In conclusion, with the lncRNA-miRNA-mRNA ceRNA network provided above, we can improve our understanding of the regulatory mechanisms and interaction among lncRNAs, miRNAs and mRNAs in TBI process.

Errataetall:

ErratumIn: Mol Brain. 2022 Jul 13;15(1):60. - PMID 35831830

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:15

Enthalten in:

Molecular brain - 15(2022), 1 vom: 02. Mai, Seite 40

Sprache:

Englisch

Beteiligte Personen:

Wang, Siqi [VerfasserIn]
Sun, Yiyu [VerfasserIn]
Hu, Shaobo [VerfasserIn]
Lou, Cen [VerfasserIn]
Pan, Yuan-Bo [VerfasserIn]

Links:

Volltext

Themen:

Bioinformatics analysis
Biomarkers, Tumor
CeRNA
Journal Article
LncRNA
MicroRNAs
Myd88
Neat1
RNA, Long Noncoding
RNA, Messenger
Research Support, Non-U.S. Gov't
Traumatic brain injury

Anmerkungen:

Date Completed 04.05.2022

Date Revised 16.07.2022

published: Electronic

ErratumIn: Mol Brain. 2022 Jul 13;15(1):60. - PMID 35831830

Citation Status MEDLINE

doi:

10.1186/s13041-022-00925-8

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM340306750