Homebrew : Protocol for glassmilk-based nucleic-acid extraction for SARS-CoV-2 diagnostics
© 2022 The Authors..
The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low-cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high-throughput settings. For complete details on the use and execution of this protocol, please refer to Page et al. (2022).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:3 |
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| Enthalten in: |
STAR protocols - 3(2022), 2 vom: 17. Juni, Seite 101300 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Page, Robert [VerfasserIn] |
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| Links: |
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| Themen: |
Immunology |
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| Anmerkungen: |
Date Completed 29.04.2022 Date Revised 28.02.2024 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.1016/j.xpro.2022.101300 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM340080043 |
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| 520 | |a © 2022 The Authors. | ||
| 520 | |a The gold standard protocol for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection detection remains reverse transcription quantitative polymerase chain reaction (qRT-PCR), which detects viral RNA more sensitively than any other approach. Here, we present Homebrew, a low-cost protocol to extract RNA using widely available reagents. Homebrew is as sensitive as commercially available RNA extraction kits. Homebrew allows for sample pooling and can be adapted for automation in high-throughput settings. For complete details on the use and execution of this protocol, please refer to Page et al. (2022) | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Immunology | |
| 650 | 4 | |a Microbiology | |
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| 700 | 1 | |a Ficarelli, Mattia |e verfasserin |4 aut | |
| 700 | 1 | |a McKellar, Stuart W |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Kwok Leung |e verfasserin |4 aut | |
| 700 | 1 | |a Maguire, Thomas J A |e verfasserin |4 aut | |
| 700 | 1 | |a Bouton, Clement |e verfasserin |4 aut | |
| 700 | 1 | |a Lista, Maria Jose |e verfasserin |4 aut | |
| 700 | 1 | |a Neil, Stuart J D |e verfasserin |4 aut | |
| 700 | 1 | |a Malim, Michael H |e verfasserin |4 aut | |
| 700 | 1 | |a Zuckerman, Mark |e verfasserin |4 aut | |
| 700 | 1 | |a Mischo, Hannah E |e verfasserin |4 aut | |
| 700 | 1 | |a Martinez-Nunez, Rocio T |e verfasserin |4 aut | |
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