The response of dual-species bacterial biofilm to 2% and 5% NaOCl mixed with etidronic acid : A laboratory real-time evaluation using optical coherence tomography
© 2022 The Authors. International Endodontic Journal published by John Wiley & Sons Ltd on behalf of British Endodontic Society..
AIM: The addition of etidronic acid (HEDP) to sodium hypochlorite (NaOCl) could increase the antibiofilm potency of the irrigant, whilst maintaining the benefits of continuous chelation. Studies conducted so far have shown that mixing HEDP with NaOCl solutions of relatively low concentration does not compromise the antibiofilm efficacy of the irrigant. However, the working lifespan of NaOCl may decrease resulting in a reduction of its antibiofilm efficacy over time (efficiency). In this regard, continuous irrigant replenishment needs to be examined. This study investigated the response of a dual-species biofilm when challenged with 2% and 5% NaOCl mixed with HEDP for a prolonged timespan and under steady laminar flow.
METHODOLOGY: Dual-species biofilms comprised of Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were grown on human dentine discs in a constant depth film fermenter (CDFF) for 96 h. Biofilms were treated with 2% and 5% NaOCl, alone or mixed with HEDP. Irrigants were applied under steady laminar flow for 8 min. Biofilm response was evaluated by means of optical coherence tomography (OCT). Biofilm removal, biofilm disruption, rate of biofilm loss and disruption as well as bubble formation were assessed. One-way anova, Wilcoxon's signed-rank test and Kruskal-Wallis H test were performed for statistical analysis of the data. The level of significance was set at a ≤.05.
RESULTS: Increasing NaOCl concentration resulted in increased biofilm removal and disruption, higher rate of biofilm loss and disruption and increased bubble formation. Mixing HEDP with NaOCl caused a delay in the antibiofilm action of the latter, without compromising its antibiofilm efficacy.
CONCLUSIONS: NaOCl concentration dictates the biofilm response irrespective of the presence of HEDP. The addition of HEDP resulted in a delay in the antibiofilm action of NaOCl. This delay affects the efficiency, but not the efficacy of the irrigant over time.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:55 |
|---|---|
| Enthalten in: |
International endodontic journal - 55(2022), 7 vom: 26. Juli, Seite 758-771 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Borges, Mariana Maciel Batista [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Biofilm |
|---|
| Anmerkungen: |
Date Completed 14.06.2022 Date Revised 31.07.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/iej.13754 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM339994770 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM339994770 | ||
| 003 | DE-627 | ||
| 005 | 20231226004139.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/iej.13754 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1133.xml |
| 035 | |a (DE-627)NLM339994770 | ||
| 035 | |a (NLM)35470434 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Borges, Mariana Maciel Batista |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The response of dual-species bacterial biofilm to 2% and 5% NaOCl mixed with etidronic acid |b A laboratory real-time evaluation using optical coherence tomography |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 14.06.2022 | ||
| 500 | |a Date Revised 31.07.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022 The Authors. International Endodontic Journal published by John Wiley & Sons Ltd on behalf of British Endodontic Society. | ||
| 520 | |a AIM: The addition of etidronic acid (HEDP) to sodium hypochlorite (NaOCl) could increase the antibiofilm potency of the irrigant, whilst maintaining the benefits of continuous chelation. Studies conducted so far have shown that mixing HEDP with NaOCl solutions of relatively low concentration does not compromise the antibiofilm efficacy of the irrigant. However, the working lifespan of NaOCl may decrease resulting in a reduction of its antibiofilm efficacy over time (efficiency). In this regard, continuous irrigant replenishment needs to be examined. This study investigated the response of a dual-species biofilm when challenged with 2% and 5% NaOCl mixed with HEDP for a prolonged timespan and under steady laminar flow | ||
| 520 | |a METHODOLOGY: Dual-species biofilms comprised of Streptococcus oralis J22 and Actinomyces naeslundii T14V-J1 were grown on human dentine discs in a constant depth film fermenter (CDFF) for 96 h. Biofilms were treated with 2% and 5% NaOCl, alone or mixed with HEDP. Irrigants were applied under steady laminar flow for 8 min. Biofilm response was evaluated by means of optical coherence tomography (OCT). Biofilm removal, biofilm disruption, rate of biofilm loss and disruption as well as bubble formation were assessed. One-way anova, Wilcoxon's signed-rank test and Kruskal-Wallis H test were performed for statistical analysis of the data. The level of significance was set at a ≤.05 | ||
| 520 | |a RESULTS: Increasing NaOCl concentration resulted in increased biofilm removal and disruption, higher rate of biofilm loss and disruption and increased bubble formation. Mixing HEDP with NaOCl caused a delay in the antibiofilm action of the latter, without compromising its antibiofilm efficacy | ||
| 520 | |a CONCLUSIONS: NaOCl concentration dictates the biofilm response irrespective of the presence of HEDP. The addition of HEDP resulted in a delay in the antibiofilm action of NaOCl. This delay affects the efficiency, but not the efficacy of the irrigant over time | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a HEDP | |
| 650 | 4 | |a biofilm | |
| 650 | 4 | |a etidronate | |
| 650 | 4 | |a irrigants | |
| 650 | 4 | |a optical coherence tomography | |
| 650 | 4 | |a sodium hypochlorite | |
| 650 | 7 | |a Root Canal Irrigants |2 NLM | |
| 650 | 7 | |a Sodium Hypochlorite |2 NLM | |
| 650 | 7 | |a DY38VHM5OD |2 NLM | |
| 650 | 7 | |a Etidronic Acid |2 NLM | |
| 650 | 7 | |a M2F465ROXU |2 NLM | |
| 700 | 1 | |a Dijkstra, René J B |e verfasserin |4 aut | |
| 700 | 1 | |a de Andrade, Flaviana Bombarda |e verfasserin |4 aut | |
| 700 | 1 | |a Duarte, Marco Antonio Hungaro |e verfasserin |4 aut | |
| 700 | 1 | |a Versluis, Michel |e verfasserin |4 aut | |
| 700 | 1 | |a van der Sluis, Lucas W M |e verfasserin |4 aut | |
| 700 | 1 | |a Petridis, Xenos |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t International endodontic journal |d 1993 |g 55(2022), 7 vom: 26. Juli, Seite 758-771 |w (DE-627)NLM012723207 |x 1365-2591 |7 nnns |
| 773 | 1 | 8 | |g volume:55 |g year:2022 |g number:7 |g day:26 |g month:07 |g pages:758-771 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/iej.13754 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 55 |j 2022 |e 7 |b 26 |c 07 |h 758-771 | ||