Serology and gene sequence analysis of the B(A) subtype of patients in Hunan
Copyright © 2022 Elsevier Ltd. All rights reserved..
INTRODUCTION: B(A) is a relatively rare ABO subtype. The frequency of B(A) in European Caucasians is low, but is higher in China. B(A) is common in northern China, gradually decreasing from north to south. The frequency of B(A) has been researched in northern China, but not in southern China. This study aimed to investigate the serological characteristics and molecular mechanism of the B(A) subtype in Hunan.
METHODS: The phenotypes of ABO blood group were analyzed by blood group serology for the patients in the Third Xiangya Hospital from 2016 to 2020; Exons 1-7 of the ABO gene were screened by PCR-SSP and sequencing.
RESULTS: Nine cases were suspected as belonging to the B(A) subtype by serological tests. The activity of D-galactosyltransferase in these patients' serum was significantly higher than that of group B plasma; meanwhile, there was no activity of Nacetylgalactosyltransferase. The ABO genotype was ABO*B.01/O.01.01 or ABO*B.01/O.01.02, and the gene sequencing results confirmed the phenotype as B(A), whose gene frequence was 1/26,000. Allele ABO*BA.02 (1/38,000) had the highest frequency, followed by ABO*BA.04 (1/77,000).
CONCLUSION: Among the patients in Hunan, the most common allele encoding the B(A) phenotype was ABO*BA.02. The identification of the B(A) blood group required serological methods combined with genotyping.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:61 |
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| Enthalten in: |
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis - 61(2022), 5 vom: 20. Okt., Seite 103449 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Liu, Fengxia [VerfasserIn] |
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| Links: |
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| Themen: |
ABO Blood-Group System |
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| Anmerkungen: |
Date Completed 25.10.2022 Date Revised 25.10.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.transci.2022.103449 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022 Elsevier Ltd. All rights reserved. | ||
| 520 | |a INTRODUCTION: B(A) is a relatively rare ABO subtype. The frequency of B(A) in European Caucasians is low, but is higher in China. B(A) is common in northern China, gradually decreasing from north to south. The frequency of B(A) has been researched in northern China, but not in southern China. This study aimed to investigate the serological characteristics and molecular mechanism of the B(A) subtype in Hunan | ||
| 520 | |a METHODS: The phenotypes of ABO blood group were analyzed by blood group serology for the patients in the Third Xiangya Hospital from 2016 to 2020; Exons 1-7 of the ABO gene were screened by PCR-SSP and sequencing | ||
| 520 | |a RESULTS: Nine cases were suspected as belonging to the B(A) subtype by serological tests. The activity of D-galactosyltransferase in these patients' serum was significantly higher than that of group B plasma; meanwhile, there was no activity of Nacetylgalactosyltransferase. The ABO genotype was ABO*B.01/O.01.01 or ABO*B.01/O.01.02, and the gene sequencing results confirmed the phenotype as B(A), whose gene frequence was 1/26,000. Allele ABO*BA.02 (1/38,000) had the highest frequency, followed by ABO*BA.04 (1/77,000) | ||
| 520 | |a CONCLUSION: Among the patients in Hunan, the most common allele encoding the B(A) phenotype was ABO*BA.02. The identification of the B(A) blood group required serological methods combined with genotyping | ||
| 650 | 4 | |a Journal Article | |
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