Assessment of DNA aptamers targeting GlcB and HspX antigens for application in the diagnosis of abdominal tuberculosis
Copyright © 2022 Elsevier Ltd. All rights reserved..
The diagnosis of abdominal tuberculosis (aTB) is challenging and there is an urgent need for an accurate diagnostic test. We have developed a high affinity DNA aptamer against GlcB antigen of Mycobacterium tuberculosis (Mtb). We further compared the diagnostic utility of in-house-generated high affinity DNA aptamers and polyclonal antibodies against two Mtb antigens, namely GlcB and HspX, in ascitic fluid samples. These diagnostic reagents were assessed in patients (n = 94) who were categorized as 'Definite TB', 'Probable TB', 'Possible TB' (taken together as aTB) and 'Non-TB' disease. Receiver operating characteristic curves were used to derive cut-off values to provide ≥93% specificity. Aptamer Linked Immobilized Sorbent Assay (ALISA) for HspX and GlcB exhibited a sensitivity of ∼84% and 50%, respectively (p-value <0.01). In contrast, antibody-based ELISA exhibited a lower sensitivity of ∼18% and ∼28% for HspX and GlcB, respectively (p-value <0.0001 and p = 0.05 for HspX and GlcB ELISA vs. ALISA, respectively). HspX ALISA detected 32/38 aTB cases, while Xpert detected only 9 samples. In conclusion, HspX aptamer-based test was found to be superior to the other tests for diagnosing aTB and it nearly fulfils the sensitivity criteria of WHO's 'Target Product Profile' for extrapulmonary tuberculosis (sensitivity ≥80%, specificity 98%).
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:134 |
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| Enthalten in: |
Tuberculosis (Edinburgh, Scotland) - 134(2022) vom: 01. Mai, Seite 102206 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kumari, Pooja [VerfasserIn] |
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| Links: |
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| Themen: |
Abdominal tuberculosis |
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| Anmerkungen: |
Date Completed 02.06.2022 Date Revised 14.06.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.tube.2022.102206 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM339914408 |
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| 520 | |a Copyright © 2022 Elsevier Ltd. All rights reserved. | ||
| 520 | |a The diagnosis of abdominal tuberculosis (aTB) is challenging and there is an urgent need for an accurate diagnostic test. We have developed a high affinity DNA aptamer against GlcB antigen of Mycobacterium tuberculosis (Mtb). We further compared the diagnostic utility of in-house-generated high affinity DNA aptamers and polyclonal antibodies against two Mtb antigens, namely GlcB and HspX, in ascitic fluid samples. These diagnostic reagents were assessed in patients (n = 94) who were categorized as 'Definite TB', 'Probable TB', 'Possible TB' (taken together as aTB) and 'Non-TB' disease. Receiver operating characteristic curves were used to derive cut-off values to provide ≥93% specificity. Aptamer Linked Immobilized Sorbent Assay (ALISA) for HspX and GlcB exhibited a sensitivity of ∼84% and 50%, respectively (p-value <0.01). In contrast, antibody-based ELISA exhibited a lower sensitivity of ∼18% and ∼28% for HspX and GlcB, respectively (p-value <0.0001 and p = 0.05 for HspX and GlcB ELISA vs. ALISA, respectively). HspX ALISA detected 32/38 aTB cases, while Xpert detected only 9 samples. In conclusion, HspX aptamer-based test was found to be superior to the other tests for diagnosing aTB and it nearly fulfils the sensitivity criteria of WHO's 'Target Product Profile' for extrapulmonary tuberculosis (sensitivity ≥80%, specificity 98%) | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Abdominal tuberculosis | |
| 650 | 4 | |a Aptamer | |
| 650 | 4 | |a Ascitic fluid | |
| 650 | 4 | |a GlcB | |
| 650 | 4 | |a TB diagnosis | |
| 650 | 4 | |a Xpert | |
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| 650 | 7 | |a Aptamers, Nucleotide |2 NLM | |
| 650 | 7 | |a Bacterial Proteins |2 NLM | |
| 700 | 1 | |a Dhiman, Abhijeet |e verfasserin |4 aut | |
| 700 | 1 | |a Lavania, Surabhi |e verfasserin |4 aut | |
| 700 | 1 | |a Sharma, Pratibha |e verfasserin |4 aut | |
| 700 | 1 | |a Rath, Deepak |e verfasserin |4 aut | |
| 700 | 1 | |a Anthwal, Divya |e verfasserin |4 aut | |
| 700 | 1 | |a Gupta, Rakesh Kumar |e verfasserin |4 aut | |
| 700 | 1 | |a Kochar, Archit |e verfasserin |4 aut | |
| 700 | 1 | |a Sharma, Neera |e verfasserin |4 aut | |
| 700 | 1 | |a Gadpayle, A K |e verfasserin |4 aut | |
| 700 | 1 | |a Taneja, R S |e verfasserin |4 aut | |
| 700 | 1 | |a Sharma, Lokesh |e verfasserin |4 aut | |
| 700 | 1 | |a Haldar, Sagarika |e verfasserin |4 aut | |
| 700 | 1 | |a Sharma, Tarun Kumar |e verfasserin |4 aut | |
| 700 | 1 | |a Tyagi, Jaya Sivaswami |e verfasserin |4 aut | |
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