The usefulness of measuring n-butyric acid concentration as a new indicator of blood decomposition in forensic autopsy
Copyright © 2022 Elsevier B.V. All rights reserved..
In forensic medicine, although various alcohols have been reported as indicators of decomposition in collected blood, no studies have examined short-chain fatty acids as indicators. In this study, the blood n-butyric acid concentration was quantified, and the association between n-butyric acid and decomposition was investigated to determine whether the detection of n-butyric acid could be a new indicator of decomposition. Among the forensic autopsies performed from 2016 to 2018 in our laboratory, the cases were divided into decomposed (n = 20) and non-decomposed (n = 20) groups based on macroscopic findings. Blood samples collected at the time of autopsy were derivatized with 3-nitrophenylhydrazine hydrochloride after solid-phase extraction. The n-butyric acid concentration was measured using liquid chromatography-tandem mass spectrometry. In addition, ethanol and n-propanol were measured using a gas chromatography-flame ionization detector. There was a significant difference (p < 0.01) in the concentrations of n-butyric acid between the decomposed and non-decomposed groups (0.343 ± 0.259 [0.030-0.973] and 0.003 ± 0.002 [0.001-0.007] mg/mL, respectively). In the decomposed group, n-butyric acid was detected at high concentrations, even in cases where n-propanol was low. These results suggest that n-butyric acid is more likely to be an indicator of blood decomposition than n-propanol.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:57 |
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| Enthalten in: |
Legal medicine (Tokyo, Japan) - 57(2022) vom: 01. Juli, Seite 102071 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Matoba, Kotaro [VerfasserIn] |
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| Links: |
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| Themen: |
1-Propanol |
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| Anmerkungen: |
Date Completed 28.06.2022 Date Revised 28.06.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.legalmed.2022.102071 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a In forensic medicine, although various alcohols have been reported as indicators of decomposition in collected blood, no studies have examined short-chain fatty acids as indicators. In this study, the blood n-butyric acid concentration was quantified, and the association between n-butyric acid and decomposition was investigated to determine whether the detection of n-butyric acid could be a new indicator of decomposition. Among the forensic autopsies performed from 2016 to 2018 in our laboratory, the cases were divided into decomposed (n = 20) and non-decomposed (n = 20) groups based on macroscopic findings. Blood samples collected at the time of autopsy were derivatized with 3-nitrophenylhydrazine hydrochloride after solid-phase extraction. The n-butyric acid concentration was measured using liquid chromatography-tandem mass spectrometry. In addition, ethanol and n-propanol were measured using a gas chromatography-flame ionization detector. There was a significant difference (p < 0.01) in the concentrations of n-butyric acid between the decomposed and non-decomposed groups (0.343 ± 0.259 [0.030-0.973] and 0.003 ± 0.002 [0.001-0.007] mg/mL, respectively). In the decomposed group, n-butyric acid was detected at high concentrations, even in cases where n-propanol was low. These results suggest that n-butyric acid is more likely to be an indicator of blood decomposition than n-propanol | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Forensic autopsy | |
| 650 | 4 | |a Liquid chromatography–tandem mass spectrometry | |
| 650 | 4 | |a Postmortem decomposition | |
| 650 | 4 | |a Postmortem production | |
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| 700 | 1 | |a Fujita, Emi |e verfasserin |4 aut | |
| 700 | 1 | |a Jin, Shigeki |e verfasserin |4 aut | |
| 700 | 1 | |a Ogasawara, Ryosuke |e verfasserin |4 aut | |
| 700 | 1 | |a Matoba, Tomoko |e verfasserin |4 aut | |
| 700 | 1 | |a Takeuchi, Akiko |e verfasserin |4 aut | |
| 700 | 1 | |a Haga, Sanae |e verfasserin |4 aut | |
| 700 | 1 | |a Ozaki, Michitaka |e verfasserin |4 aut | |
| 700 | 1 | |a Hyodoh, Hideki |e verfasserin |4 aut | |
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