Whole-genome sequence of a putative pathogenic Bacillus sp. strain SD-4 isolated from cattle feed

Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved..

OBJECTIVES: The present study describes the draft genome sequence of a novel Bacillus sp. strain SD-4 isolated from animal feed. The study aims to get a deeper insight into antimicrobial resistance and secondary metabolite biosynthetic gene clusters (BGCs) and the association between them.

METHODS: The strain SD-4 was preliminarily evaluated for antibacterial activities, motility, biofilm formation, and enterotoxin production using in vitro assays. The genome of strain SD-4 was sequenced using the Illumina HiSeq 2500 platform with paired-end reads. The reads were assembled and annotated using SPAdes and PGAP, respectively. The genome was further analysed using several bioinformatics tools, including TYGS, AntiSMASH, RAST, PlasmidFinder, VFDB, VirulenceFinder, CARD, PathogenFinder, MobileElement finder, IslandViewer, and CRISPRFinder.

RESULTS: In vitro assays showed that the strain is motile, synthesises biofilm, and produces an enterotoxin and antibacterial metabolites. The genome analysis revealed that the strain SD-4 carries antimicrobial resistance genes (ARGs), virulence factors, and beneficial secondary metabolite BGCs. Further genome analysis showed interesting genome architectures containing several mobile genetic elements, including two plasmid replicons (repUS22 and rep20), five prophages, and at least four genomic islands (GIs), including one Listeria pathogenicity island LIPI-1. Moreover, the strain SD-4 is identified as a putative human pathogen.

CONCLUSION: The genome of strain SD-4 harbours several BGCs coding for biologically active metabolites. It also contains antimicrobial resistance genes and is identified as a potential human pathogen. These results can be used to better comprehend antibiotic resistance in environmental bacteria that are not influenced by human intervention.

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:29

Enthalten in:

Journal of global antimicrobial resistance - 29(2022) vom: 15. Juni, Seite 293-295

Sprache:

Englisch

Beteiligte Personen:

Iqbal, Sajid [VerfasserIn]
Bhatti, Muhammad Faraz [VerfasserIn]
Javed, Aneela [VerfasserIn]
Rahim, Kashif [VerfasserIn]
Paracha, Rehan Zafar [VerfasserIn]
Janjua, Hussnain Ahmed [VerfasserIn]

Links:

Volltext

Themen:

Anti-Bacterial Agents
Antimicrobial resistance genes
Bacillus sp
Enterotoxins
Journal Article
Mobile genetic elements
Research Support, Non-U.S. Gov't
SD-4
Secondary metabolites biosynthetic gene clusters
Virulence genes

Anmerkungen:

Date Completed 21.06.2022

Date Revised 03.10.2022

published: Print-Electronic

Citation Status MEDLINE

doi:

10.1016/j.jgar.2022.04.002

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM339429909