Human pluripotent stem cell-derived myogenic progenitors undergo maturation to quiescent satellite cells upon engraftment
Copyright © 2022 Elsevier Inc. All rights reserved..
GFP), we demonstrate that hPSC-derived MPCs can contribute to the regeneration of myofibers in mice following local injury and in mice deficient of dystrophin (mdx). We also demonstrate that a subset of PAX7::GFP MPCs engraft within the basal lamina of regenerated myofibers, adopt a quiescent state, and contribute to regeneration upon reinjury and in mdx mouse models. This subset of PAX7::GFP MPCs undergo a maturation process and remodel their molecular characteristics to resemble those of late-stage fetal MPCs/adult satellite cells following in vivo engraftment. These in-vivo-matured PAX7::GFP MPCs retain a cell-autonomous ability to regenerate and can repopulate in the niche of secondary recipient mice, providing a proof of principle for future hPSC-based cell therapy for muscle disorders.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:29 |
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| Enthalten in: |
Cell stem cell - 29(2022), 4 vom: 07. Apr., Seite 610-619.e5 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Sun, Congshan [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 12.04.2022 Date Revised 26.05.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1016/j.stem.2022.03.004 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Human pluripotent stem cell-derived myogenic progenitors undergo maturation to quiescent satellite cells upon engraftment |
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| 520 | |a Human pluripotent stem cell (hPSC)-derived myogenic progenitor cell (MPC) transplantation is a promising therapeutic approach for a variety of degenerative muscle disorders. Here, using an MPC-specific fluorescent reporter system (PAX7::GFP), we demonstrate that hPSC-derived MPCs can contribute to the regeneration of myofibers in mice following local injury and in mice deficient of dystrophin (mdx). We also demonstrate that a subset of PAX7::GFP MPCs engraft within the basal lamina of regenerated myofibers, adopt a quiescent state, and contribute to regeneration upon reinjury and in mdx mouse models. This subset of PAX7::GFP MPCs undergo a maturation process and remodel their molecular characteristics to resemble those of late-stage fetal MPCs/adult satellite cells following in vivo engraftment. These in-vivo-matured PAX7::GFP MPCs retain a cell-autonomous ability to regenerate and can repopulate in the niche of secondary recipient mice, providing a proof of principle for future hPSC-based cell therapy for muscle disorders | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Duchenne muscular dystrophy | |
| 650 | 4 | |a mdx mouse | |
| 650 | 4 | |a pluripotent stem cells | |
| 650 | 4 | |a quiescent stem cells | |
| 650 | 4 | |a skeletal muscle stem cells | |
| 650 | 7 | |a Dystrophin |2 NLM | |
| 700 | 1 | |a Kannan, Suraj |e verfasserin |4 aut | |
| 700 | 1 | |a Choi, In Young |e verfasserin |4 aut | |
| 700 | 1 | |a Lim, HoTae |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Hao |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Grace S |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Nancy |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Seong-Hyun |e verfasserin |4 aut | |
| 700 | 1 | |a Serra, Carlo |e verfasserin |4 aut | |
| 700 | 1 | |a Iyer, Shama R |e verfasserin |4 aut | |
| 700 | 1 | |a Lloyd, Thomas E |e verfasserin |4 aut | |
| 700 | 1 | |a Kwon, Chulan |e verfasserin |4 aut | |
| 700 | 1 | |a Lovering, Richard M |e verfasserin |4 aut | |
| 700 | 1 | |a Lim, Su Bin |e verfasserin |4 aut | |
| 700 | 1 | |a Andersen, Peter |e verfasserin |4 aut | |
| 700 | 1 | |a Wagner, Kathryn R |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Gabsang |e verfasserin |4 aut | |
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