Antigen-Presenting B Cells Program the Efferent Lymph T Helper Cell Response
Copyright © 2022 Alsén, Cervin, Deng, Szeponik, Wenzel, Karlsson, Cucak, Livingston, Bryder, Lu, Johansson-Lindbom and Yrlid..
B cells interact with T follicular helper (Tfh) cells in germinal centers (GCs) to generate high-affinity antibodies. Much less is known about how cognate T-B-cell interactions influence Th cells that enter circulation and peripheral tissues. Therefore, we generated mice lacking MHC-II expressing B cells and, by thoracic duct cannulation, analyzed Th cells in the efferent lymph at defined intervals post-immunization. Focusing on gut-draining mesenteric lymph nodes (MLNs), we show that antigen-specific α4β7+ gut-homing effector Th cells enter the circulation prior to CXCR5+PD-1+ Tfh-like cells. B cells appear to have no or limited impact on the early generation and egress of gut-homing Th cells but are critical for the subsequent appearance of Tfh-like cells that peak in the lymph before GCs have developed. At this stage, antigen-presenting B cells also reduce the proportion of α4β7+ Th cells in the MLN and efferent lymph. Furthermore, cognate B-cell interaction drives a broad transcriptional program in Th cells, including IL-4 that is confined to the Tfh cell lineage. The IL-4-producing Tfh-like cells originate from Bcl6+ precursors in the LNs and have gut-homing capacity. Hence, B cells program the efferent lymph Th cell response within a limited window of time after antigenic challenge.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Frontiers in immunology - 13(2022) vom: 18., Seite 813203 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Alsén, Samuel [VerfasserIn] |
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| Links: |
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| Themen: |
207137-56-2 |
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| Anmerkungen: |
Date Completed 08.04.2022 Date Revised 20.05.2022 published: Electronic-eCollection Citation Status MEDLINE |
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| doi: |
10.3389/fimmu.2022.813203 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a B cells interact with T follicular helper (Tfh) cells in germinal centers (GCs) to generate high-affinity antibodies. Much less is known about how cognate T-B-cell interactions influence Th cells that enter circulation and peripheral tissues. Therefore, we generated mice lacking MHC-II expressing B cells and, by thoracic duct cannulation, analyzed Th cells in the efferent lymph at defined intervals post-immunization. Focusing on gut-draining mesenteric lymph nodes (MLNs), we show that antigen-specific α4β7+ gut-homing effector Th cells enter the circulation prior to CXCR5+PD-1+ Tfh-like cells. B cells appear to have no or limited impact on the early generation and egress of gut-homing Th cells but are critical for the subsequent appearance of Tfh-like cells that peak in the lymph before GCs have developed. At this stage, antigen-presenting B cells also reduce the proportion of α4β7+ Th cells in the MLN and efferent lymph. Furthermore, cognate B-cell interaction drives a broad transcriptional program in Th cells, including IL-4 that is confined to the Tfh cell lineage. The IL-4-producing Tfh-like cells originate from Bcl6+ precursors in the LNs and have gut-homing capacity. Hence, B cells program the efferent lymph Th cell response within a limited window of time after antigenic challenge | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a B cells | |
| 650 | 4 | |a T cells | |
| 650 | 4 | |a efferent lymph | |
| 650 | 4 | |a gut-homing CD4+ T cells | |
| 650 | 4 | |a small intestinal lamina propria | |
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| 700 | 1 | |a Deng, Yaxiong |e verfasserin |4 aut | |
| 700 | 1 | |a Szeponik, Louis |e verfasserin |4 aut | |
| 700 | 1 | |a Wenzel, Ulf Alexander |e verfasserin |4 aut | |
| 700 | 1 | |a Karlsson, Joakim |e verfasserin |4 aut | |
| 700 | 1 | |a Cucak, Helena |e verfasserin |4 aut | |
| 700 | 1 | |a Livingston, Megan |e verfasserin |4 aut | |
| 700 | 1 | |a Bryder, David |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Qianjin |e verfasserin |4 aut | |
| 700 | 1 | |a Johansson-Lindbom, Bengt |e verfasserin |4 aut | |
| 700 | 1 | |a Yrlid, Ulf |e verfasserin |4 aut | |
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