Shared signatures and divergence in skin microbiomes of children with atopic dermatitis and their caregivers
Copyright © 2022 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved..
BACKGROUND: Atopic dermatitis (AD) is a common chronic skin condition in children (15-20%) that can significantly impair their quality of life. As a result of its relapsing nature and enrichment of Staphylococcus aureus during flares, clinical management can include eradicating S aureus from the skin of children; however, this does not extend to their healthy caregivers, who are potential reservoirs.
OBJECTIVE: Our aim was to understand skin microbiome sharing and microbial features in children with AD and their healthy adult caregivers.
METHODS: We utilized whole-metagenome profiling at 4 body sites (volar forearm, antecubital fossae, cheeks, and lesions) in combination with sequencing of S aureus isolates to characterize a cohort of children with AD and their healthy caregivers (n = 30 families) compared to matched pairs from control households (n = 30 families).
RESULTS: Metagenomic analysis revealed distinct microbiome configurations in the nonlesional skin of AD children and their healthy caregivers versus controls, which were sufficient to accurately predict case-control status (area under the receiver operating characteristic curve > 0.8). These differences were accompanied by significant microbiome similarity between children and their caregivers, indicating that microbiome sharing may play a role in recurrent disease flares. Whole-genome comparisons with high-quality S aureus isolate genomes (n = 55) confirmed significant strain sharing between AD children and their caregivers and AD-specific enrichment of strains expressing enterotoxins Q and K/K2.
CONCLUSION: Our results highlight the distinctive skin microbiome features of healthy caregivers for children with AD and support their inclusion in strategies for the treatment of recurrent pediatric AD.
| Errataetall: |
CommentIn: J Allergy Clin Immunol. 2022 Oct;150(4):793-795. - PMID 35931225 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:150 |
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| Enthalten in: |
The Journal of allergy and clinical immunology - 150(2022), 4 vom: 25. Okt., Seite 894-908 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chia, Minghao [VerfasserIn] |
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| Links: |
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| Themen: |
Atopic dermatitis |
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| Anmerkungen: |
Date Completed 10.10.2022 Date Revised 26.10.2022 published: Print-Electronic CommentIn: J Allergy Clin Immunol. 2022 Oct;150(4):793-795. - PMID 35931225 Citation Status MEDLINE |
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| doi: |
10.1016/j.jaci.2022.01.031 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 500 | |a Date Revised 26.10.2022 | ||
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| 500 | |a CommentIn: J Allergy Clin Immunol. 2022 Oct;150(4):793-795. - PMID 35931225 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a BACKGROUND: Atopic dermatitis (AD) is a common chronic skin condition in children (15-20%) that can significantly impair their quality of life. As a result of its relapsing nature and enrichment of Staphylococcus aureus during flares, clinical management can include eradicating S aureus from the skin of children; however, this does not extend to their healthy caregivers, who are potential reservoirs | ||
| 520 | |a OBJECTIVE: Our aim was to understand skin microbiome sharing and microbial features in children with AD and their healthy adult caregivers | ||
| 520 | |a METHODS: We utilized whole-metagenome profiling at 4 body sites (volar forearm, antecubital fossae, cheeks, and lesions) in combination with sequencing of S aureus isolates to characterize a cohort of children with AD and their healthy caregivers (n = 30 families) compared to matched pairs from control households (n = 30 families) | ||
| 520 | |a RESULTS: Metagenomic analysis revealed distinct microbiome configurations in the nonlesional skin of AD children and their healthy caregivers versus controls, which were sufficient to accurately predict case-control status (area under the receiver operating characteristic curve > 0.8). These differences were accompanied by significant microbiome similarity between children and their caregivers, indicating that microbiome sharing may play a role in recurrent disease flares. Whole-genome comparisons with high-quality S aureus isolate genomes (n = 55) confirmed significant strain sharing between AD children and their caregivers and AD-specific enrichment of strains expressing enterotoxins Q and K/K2 | ||
| 520 | |a CONCLUSION: Our results highlight the distinctive skin microbiome features of healthy caregivers for children with AD and support their inclusion in strategies for the treatment of recurrent pediatric AD | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Atopic dermatitis | |
| 650 | 4 | |a Staphylococcus aureus | |
| 650 | 4 | |a caregivers | |
| 650 | 4 | |a household contact | |
| 650 | 4 | |a skin microbiome | |
| 650 | 7 | |a Enterotoxins |2 NLM | |
| 700 | 1 | |a Naim, Ahmad N M |e verfasserin |4 aut | |
| 700 | 1 | |a Tay, Angeline S L |e verfasserin |4 aut | |
| 700 | 1 | |a Lim, Karmun |e verfasserin |4 aut | |
| 700 | 1 | |a Chew, Kean Lee |e verfasserin |4 aut | |
| 700 | 1 | |a Yow, See Jie |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, John |e verfasserin |4 aut | |
| 700 | 1 | |a Common, John E A |e verfasserin |4 aut | |
| 700 | 1 | |a Nagarajan, Niranjan |e verfasserin |4 aut | |
| 700 | 1 | |a Tham, Elizabeth Huiwen |e verfasserin |4 aut | |
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