Tether-free photothermal deep-brain stimulation in freely behaving mice via wide-field illumination in the near-infrared-II window
© 2022. The Author(s), under exclusive licence to Springer Nature Limited..
Neural circuitry is typically modulated via invasive brain implants and tethered optical fibres in restrained animals. Here we show that wide-field illumination in the second near-infrared spectral window (NIR-II) enables implant-and-tether-free deep-brain stimulation in freely behaving mice with stereotactically injected macromolecular photothermal transducers activating neurons ectopically expressing the temperature-sensitive transient receptor potential cation channel subfamily V member 1 (TRPV1). The macromolecular transducers, ~40 nm in size and consisting of a semiconducting polymer core and an amphiphilic polymer shell, have a photothermal conversion efficiency of 71% at 1,064 nm, the wavelength at which light attenuation by brain tissue is minimized (within the 400-1,800 nm spectral window). TRPV1-expressing neurons in the hippocampus, motor cortex and ventral tegmental area of mice can be activated with minimal thermal damage on wide-field NIR-II illumination from a light source placed at distances higher than 50 cm above the animal's head and at an incident power density of 10 mW mm-2. Deep-brain stimulation via wide-field NIR-II illumination may open up opportunities for social behavioural studies in small animals.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:6 |
|---|---|
| Enthalten in: |
Nature biomedical engineering - 6(2022), 6 vom: 21. Juni, Seite 754-770 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wu, Xiang [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Journal Article |
|---|
| Anmerkungen: |
Date Completed 23.06.2022 Date Revised 12.11.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1038/s41551-022-00862-w |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM338452303 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM338452303 | ||
| 003 | DE-627 | ||
| 005 | 20231226000716.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231226s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1038/s41551-022-00862-w |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1128.xml |
| 035 | |a (DE-627)NLM338452303 | ||
| 035 | |a (NLM)35314800 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wu, Xiang |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Tether-free photothermal deep-brain stimulation in freely behaving mice via wide-field illumination in the near-infrared-II window |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 23.06.2022 | ||
| 500 | |a Date Revised 12.11.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022. The Author(s), under exclusive licence to Springer Nature Limited. | ||
| 520 | |a Neural circuitry is typically modulated via invasive brain implants and tethered optical fibres in restrained animals. Here we show that wide-field illumination in the second near-infrared spectral window (NIR-II) enables implant-and-tether-free deep-brain stimulation in freely behaving mice with stereotactically injected macromolecular photothermal transducers activating neurons ectopically expressing the temperature-sensitive transient receptor potential cation channel subfamily V member 1 (TRPV1). The macromolecular transducers, ~40 nm in size and consisting of a semiconducting polymer core and an amphiphilic polymer shell, have a photothermal conversion efficiency of 71% at 1,064 nm, the wavelength at which light attenuation by brain tissue is minimized (within the 400-1,800 nm spectral window). TRPV1-expressing neurons in the hippocampus, motor cortex and ventral tegmental area of mice can be activated with minimal thermal damage on wide-field NIR-II illumination from a light source placed at distances higher than 50 cm above the animal's head and at an incident power density of 10 mW mm-2. Deep-brain stimulation via wide-field NIR-II illumination may open up opportunities for social behavioural studies in small animals | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, U.S. Gov't, Non-P.H.S. | |
| 650 | 7 | |a Polymers |2 NLM | |
| 700 | 1 | |a Jiang, Yuyan |e verfasserin |4 aut | |
| 700 | 1 | |a Rommelfanger, Nicholas J |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Fan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Yin, Rongkang |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Junlang |e verfasserin |4 aut | |
| 700 | 1 | |a Cai, Sa |e verfasserin |4 aut | |
| 700 | 1 | |a Ren, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Shin, Andrew |e verfasserin |4 aut | |
| 700 | 1 | |a Ong, Kyrstyn S |e verfasserin |4 aut | |
| 700 | 1 | |a Pu, Kanyi |e verfasserin |4 aut | |
| 700 | 1 | |a Hong, Guosong |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Nature biomedical engineering |d 2016 |g 6(2022), 6 vom: 21. Juni, Seite 754-770 |w (DE-627)NLM271447966 |x 2157-846X |7 nnns |
| 773 | 1 | 8 | |g volume:6 |g year:2022 |g number:6 |g day:21 |g month:06 |g pages:754-770 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1038/s41551-022-00862-w |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 6 |j 2022 |e 6 |b 21 |c 06 |h 754-770 | ||