The Effect of the Nanoparticle Shape on T Cell Activation
© 2022 Wiley-VCH GmbH..
The mechanism of extracellular ligand nano-geometry in ex vivo T cell activation for immunotherapy remains elusive. Herein, the authors demonstrate large aspect ratio (AR) of gold nanorods (AuNRs) conjugated on cell culture substrate enhancing both murine and human T cell activation through the nanoscale anisotropic presentation of stimulatory ligands (anti-CD3(αCD3) and anti-CD28(αCD28) antibodies). AuNRs with large AR bearing αCD3 and αCD28 antibodies significantly promote T cell expansion and key cytokine secretion including interleukin-2 (IL-2), interferon-gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α). High membrane tension observed in large AR AuNRs regulates actin filament and focal adhesion assembly and develops maturation-related morphological features in T cells such as membrane ruffle formation, cell spreading, and large T cell receptor (TCR) cluster formation. Anisotropic stimulatory ligand presentation promotes differentiation of naïve CD8+ T cells toward the effector phenotype inducing CD137 expression upon co-culture with human cervical carcinoma. The findings suggest the importance of manipulating extracellular ligand nano-geometry in optimizing T cell behaviors to enhance therapeutic outcomes.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:18 |
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| Enthalten in: |
Small (Weinheim an der Bergstrasse, Germany) - 18(2022), 36 vom: 27. Sept., Seite e2107373 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Oh, Jiwon [VerfasserIn] |
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| Links: |
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| Themen: |
Anisotropic nanomaterials |
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| Anmerkungen: |
Date Completed 09.09.2022 Date Revised 19.10.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1002/smll.202107373 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM338278303 |
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| 520 | |a The mechanism of extracellular ligand nano-geometry in ex vivo T cell activation for immunotherapy remains elusive. Herein, the authors demonstrate large aspect ratio (AR) of gold nanorods (AuNRs) conjugated on cell culture substrate enhancing both murine and human T cell activation through the nanoscale anisotropic presentation of stimulatory ligands (anti-CD3(αCD3) and anti-CD28(αCD28) antibodies). AuNRs with large AR bearing αCD3 and αCD28 antibodies significantly promote T cell expansion and key cytokine secretion including interleukin-2 (IL-2), interferon-gamma (IFN-γ), and tumor necrosis factor-alpha (TNF-α). High membrane tension observed in large AR AuNRs regulates actin filament and focal adhesion assembly and develops maturation-related morphological features in T cells such as membrane ruffle formation, cell spreading, and large T cell receptor (TCR) cluster formation. Anisotropic stimulatory ligand presentation promotes differentiation of naïve CD8+ T cells toward the effector phenotype inducing CD137 expression upon co-culture with human cervical carcinoma. The findings suggest the importance of manipulating extracellular ligand nano-geometry in optimizing T cell behaviors to enhance therapeutic outcomes | ||
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| 650 | 4 | |a T cell activation | |
| 650 | 4 | |a anisotropic nanomaterials | |
| 650 | 4 | |a cell membrane tension | |
| 650 | 4 | |a nanoscale ligand presentation | |
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| 700 | 1 | |a Xia, Xingyu |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Wai Ki Ricky |e verfasserin |4 aut | |
| 700 | 1 | |a Wong, Siu Hong Dexter |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Weihao |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Haixing |e verfasserin |4 aut | |
| 700 | 1 | |a Lai, Chun Him Nathanael |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Ye |e verfasserin |4 aut | |
| 700 | 1 | |a Ho, Yi-Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Honglu |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Gang |e verfasserin |4 aut | |
| 700 | 1 | |a Lin, Yuan |e verfasserin |4 aut | |
| 700 | 1 | |a Bian, Liming |e verfasserin |4 aut | |
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