Effects and mechanism of miR-133a on invasion and migration of lung cancer cells
AJTR Copyright © 2022..
OBJECTIVES: To study the role of miR-133a expression in the invasion, proliferation, migration, and apoptosis of lung cancer cells and its mechanism.
METHODS: miR-133a expression levels in human normal lung epithelial cells (BEAS-2B), H441 cell lines and NSCLC tissues were detected by qPCR. The influence of miR-133a mimics on the migration, proliferation and invasion of H441 cells was examined by CCK-8 assay, transwell migration assay, and invasion assay, respectively. Expression of MMP-9 and LASP1 in H441 cellstreated by miR-133a mimics was determined by western blot. Pearson's test was conducted to study the association of miR-133a expression with clinical characteristics of NSCLC patients. The targeted regulation of miR-133a on LASP1 gene expression was detected by the luciferase reporter gene assay.
RESULTS: miR-133a expression was decreased in H441 cells in contrast to that in BEAS-2B cells (P<0.05). Compared with para-carcinoma tissues, miR-133a levels were markedly down-regulated in NSCLC tissues. miR-133a overexpression inhibited the invasion, proliferation, and migration ability of H441 cells and promoted cell apoptosis (all P<0.05). MMP-9 expression levels were also reduced in the miR-133a mimic group. Moreover, miR-133a expression levels were correlated with tumor size and TNM stage. miR-133a overexpression decreased the expression of LASP1, which is the targeted gene of miR-133a.
CONCLUSIONS: miR-133a overexpression can reduce the invasion, proliferation, migration, and matrix metalloproteinase expression of NSCLC cells and promote cell apoptosis. This may be correlated to targeted down-regulation of LASP1 expression.
| Medienart: |
Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
American journal of translational research - 14(2022), 2 vom: 03., Seite 728-739 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Yu, Bing [VerfasserIn] |
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| Themen: |
Invasion |
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| Anmerkungen: |
Date Revised 14.03.2022 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM338045171 |
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| 245 | 1 | 0 | |a Effects and mechanism of miR-133a on invasion and migration of lung cancer cells |
| 264 | 1 | |c 2022 | |
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| 500 | |a Date Revised 14.03.2022 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a AJTR Copyright © 2022. | ||
| 520 | |a OBJECTIVES: To study the role of miR-133a expression in the invasion, proliferation, migration, and apoptosis of lung cancer cells and its mechanism | ||
| 520 | |a METHODS: miR-133a expression levels in human normal lung epithelial cells (BEAS-2B), H441 cell lines and NSCLC tissues were detected by qPCR. The influence of miR-133a mimics on the migration, proliferation and invasion of H441 cells was examined by CCK-8 assay, transwell migration assay, and invasion assay, respectively. Expression of MMP-9 and LASP1 in H441 cellstreated by miR-133a mimics was determined by western blot. Pearson's test was conducted to study the association of miR-133a expression with clinical characteristics of NSCLC patients. The targeted regulation of miR-133a on LASP1 gene expression was detected by the luciferase reporter gene assay | ||
| 520 | |a RESULTS: miR-133a expression was decreased in H441 cells in contrast to that in BEAS-2B cells (P<0.05). Compared with para-carcinoma tissues, miR-133a levels were markedly down-regulated in NSCLC tissues. miR-133a overexpression inhibited the invasion, proliferation, and migration ability of H441 cells and promoted cell apoptosis (all P<0.05). MMP-9 expression levels were also reduced in the miR-133a mimic group. Moreover, miR-133a expression levels were correlated with tumor size and TNM stage. miR-133a overexpression decreased the expression of LASP1, which is the targeted gene of miR-133a | ||
| 520 | |a CONCLUSIONS: miR-133a overexpression can reduce the invasion, proliferation, migration, and matrix metalloproteinase expression of NSCLC cells and promote cell apoptosis. This may be correlated to targeted down-regulation of LASP1 expression | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a NSCLC | |
| 650 | 4 | |a invasion | |
| 650 | 4 | |a matrix metalloproteinase | |
| 650 | 4 | |a miR-133a | |
| 650 | 4 | |a migration | |
| 650 | 4 | |a proliferation | |
| 700 | 1 | |a Pang, Jinghua |e verfasserin |4 aut | |
| 700 | 1 | |a You, Jiawen |e verfasserin |4 aut | |
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