Homebrew : An economical and sensitive glassmilk-based nucleic-acid extraction method for SARS-CoV-2 diagnostics
© 2022 The Authors..
Management of COVID-19 and other epidemics requires large-scale diagnostic testing. The gold standard for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains reverse transcription quantitative PCR (qRT-PCR) analysis, which detects viral RNA more sensitively than any other method. However, the resource use and supply-chain requirements of RT-PCR have continued to challenge diagnostic laboratories worldwide. Here, we establish and characterize a low-cost method to detect SARS-CoV-2 in clinical combined nose and throat swabs, allowing for automation in high-throughput settings. This method inactivates virus material with sodium dodecylsulfate (SDS) and uses silicon dioxide as the RNA-binding matrix in combination with sodium chloride (NaCl) and isopropanol. With similar sensitivity for SARS-CoV-2 viral targets but a fraction of time and reagent expenditure compared with commercial kits, our method also enables sample pooling without loss of sensitivity. We suggest that this method will facilitate more economical widespread testing, particularly in resource-limited settings.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:2 |
|---|---|
| Enthalten in: |
Cell reports methods - 2(2022), 3 vom: 28. März, Seite 100186 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Page, Robert [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
COVID-19 |
|---|
| Anmerkungen: |
Date Completed 25.09.2023 Date Revised 28.02.2024 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.crmeth.2022.100186 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM337929440 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM337929440 | ||
| 003 | DE-627 | ||
| 005 | 20240229165904.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.crmeth.2022.100186 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1310.xml |
| 035 | |a (DE-627)NLM337929440 | ||
| 035 | |a (NLM)35262039 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Page, Robert |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Homebrew |b An economical and sensitive glassmilk-based nucleic-acid extraction method for SARS-CoV-2 diagnostics |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 25.09.2023 | ||
| 500 | |a Date Revised 28.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022 The Authors. | ||
| 520 | |a Management of COVID-19 and other epidemics requires large-scale diagnostic testing. The gold standard for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection remains reverse transcription quantitative PCR (qRT-PCR) analysis, which detects viral RNA more sensitively than any other method. However, the resource use and supply-chain requirements of RT-PCR have continued to challenge diagnostic laboratories worldwide. Here, we establish and characterize a low-cost method to detect SARS-CoV-2 in clinical combined nose and throat swabs, allowing for automation in high-throughput settings. This method inactivates virus material with sodium dodecylsulfate (SDS) and uses silicon dioxide as the RNA-binding matrix in combination with sodium chloride (NaCl) and isopropanol. With similar sensitivity for SARS-CoV-2 viral targets but a fraction of time and reagent expenditure compared with commercial kits, our method also enables sample pooling without loss of sensitivity. We suggest that this method will facilitate more economical widespread testing, particularly in resource-limited settings | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a COVID-19 | |
| 650 | 4 | |a SARS-CoV-2 | |
| 650 | 4 | |a low-cost RNA extraction | |
| 650 | 4 | |a molecular diagnostics | |
| 650 | 4 | |a qPCR diagnostics | |
| 700 | 1 | |a Scourfield, Edward |e verfasserin |4 aut | |
| 700 | 1 | |a Ficarelli, Mattia |e verfasserin |4 aut | |
| 700 | 1 | |a McKellar, Stuart W |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Kwok Leung |e verfasserin |4 aut | |
| 700 | 1 | |a Maguire, Thomas J A |e verfasserin |4 aut | |
| 700 | 1 | |a Bouton, Clement |e verfasserin |4 aut | |
| 700 | 1 | |a Lista, Maria Jose |e verfasserin |4 aut | |
| 700 | 1 | |a Neil, Stuart J D |e verfasserin |4 aut | |
| 700 | 1 | |a Malim, Michael H |e verfasserin |4 aut | |
| 700 | 1 | |a Zuckerman, Mark |e verfasserin |4 aut | |
| 700 | 1 | |a Mischo, Hannah E |e verfasserin |4 aut | |
| 700 | 1 | |a Martinez-Nunez, Rocio T |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Cell reports methods |d 2021 |g 2(2022), 3 vom: 28. März, Seite 100186 |w (DE-627)NLM327814853 |x 2667-2375 |7 nnns |
| 773 | 1 | 8 | |g volume:2 |g year:2022 |g number:3 |g day:28 |g month:03 |g pages:100186 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.crmeth.2022.100186 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 2 |j 2022 |e 3 |b 28 |c 03 |h 100186 | ||