Practical Assessment of an Interdisciplinary Bacteriophage Delivery Pipeline for Personalized Therapy of Gram-Negative Bacterial Infections
Despite numerous advances in personalized phage therapy, smooth logistics are challenging, particularly for multidrug-resistant Gram-negative bacterial infections requiring high numbers of specific lytic phages. We conducted this study to pave the way for efficient logistics for critically ill patients by (1) closely examining and improving a current pipeline under realistic conditions, (2) offering guidelines for each step, leading to safe and high-quality phage supplies, and (3) providing a tool to evaluate the pipeline's efficiency. Due to varying stipulations for quality and safety in different countries, we focused the pipeline on all steps up to a required phage product by a cell-free extract system. The first of three study runs included patients with respiratory bacterial infections from four intensive care units, and it revealed a cumulative time of up to 23 days. Ultimately, adjustment of specific set points of the vulnerable components of the pipeline, phage isolation, and titration increased the pipeline's efficiency by 15% and decreased the maximum required time to 13 days. We present a site-independent practical approach to establish and optimize pipelines for personalized phage delivery, the co-organization of pipeline components between different institutions, non-binding guidelines for every step, and an efficiency check for phage laboratories.
Medienart: |
E-Artikel |
---|
Erscheinungsjahr: |
2022 |
---|---|
Erschienen: |
2022 |
Enthalten in: |
Zur Gesamtaufnahme - volume:15 |
---|---|
Enthalten in: |
Pharmaceuticals (Basel, Switzerland) - 15(2022), 2 vom: 02. Feb. |
Sprache: |
Englisch |
---|
Beteiligte Personen: |
Würstle, Silvia [VerfasserIn] |
---|
Links: |
---|
Themen: |
Antimicrobial resistance |
---|
Anmerkungen: |
Date Revised 01.03.2022 published: Electronic Citation Status PubMed-not-MEDLINE |
---|
doi: |
10.3390/ph15020186 |
---|
funding: |
|
---|---|
Förderinstitution / Projekttitel: |
|
PPN (Katalog-ID): |
NLM337468079 |
---|
LEADER | 01000naa a22002652 4500 | ||
---|---|---|---|
001 | NLM337468079 | ||
003 | DE-627 | ||
005 | 20231225234456.0 | ||
007 | cr uuu---uuuuu | ||
008 | 231225s2022 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.3390/ph15020186 |2 doi | |
028 | 5 | 2 | |a pubmed24n1124.xml |
035 | |a (DE-627)NLM337468079 | ||
035 | |a (NLM)35215298 | ||
035 | |a (PII)186 | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
100 | 1 | |a Würstle, Silvia |e verfasserin |4 aut | |
245 | 1 | 0 | |a Practical Assessment of an Interdisciplinary Bacteriophage Delivery Pipeline for Personalized Therapy of Gram-Negative Bacterial Infections |
264 | 1 | |c 2022 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ƒaComputermedien |b c |2 rdamedia | ||
338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
500 | |a Date Revised 01.03.2022 | ||
500 | |a published: Electronic | ||
500 | |a Citation Status PubMed-not-MEDLINE | ||
520 | |a Despite numerous advances in personalized phage therapy, smooth logistics are challenging, particularly for multidrug-resistant Gram-negative bacterial infections requiring high numbers of specific lytic phages. We conducted this study to pave the way for efficient logistics for critically ill patients by (1) closely examining and improving a current pipeline under realistic conditions, (2) offering guidelines for each step, leading to safe and high-quality phage supplies, and (3) providing a tool to evaluate the pipeline's efficiency. Due to varying stipulations for quality and safety in different countries, we focused the pipeline on all steps up to a required phage product by a cell-free extract system. The first of three study runs included patients with respiratory bacterial infections from four intensive care units, and it revealed a cumulative time of up to 23 days. Ultimately, adjustment of specific set points of the vulnerable components of the pipeline, phage isolation, and titration increased the pipeline's efficiency by 15% and decreased the maximum required time to 13 days. We present a site-independent practical approach to establish and optimize pipelines for personalized phage delivery, the co-organization of pipeline components between different institutions, non-binding guidelines for every step, and an efficiency check for phage laboratories | ||
650 | 4 | |a Journal Article | |
650 | 4 | |a COVID-19 superinfection | |
650 | 4 | |a Klebsiella spp. | |
650 | 4 | |a antimicrobial resistance | |
650 | 4 | |a bacteriophage | |
650 | 4 | |a cell-free extract | |
650 | 4 | |a in vitro phage production | |
650 | 4 | |a phage therapy | |
700 | 1 | |a Stender, Jana |e verfasserin |4 aut | |
700 | 1 | |a Hammerl, Jens André |e verfasserin |4 aut | |
700 | 1 | |a Vogele, Kilian |e verfasserin |4 aut | |
700 | 1 | |a Rothe, Kathrin |e verfasserin |4 aut | |
700 | 1 | |a Willy, Christian |e verfasserin |4 aut | |
700 | 1 | |a Bugert, Joachim Jakob |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Pharmaceuticals (Basel, Switzerland) |d 2009 |g 15(2022), 2 vom: 02. Feb. |w (DE-627)NLM199619514 |x 1424-8247 |7 nnns |
773 | 1 | 8 | |g volume:15 |g year:2022 |g number:2 |g day:02 |g month:02 |
856 | 4 | 0 | |u http://dx.doi.org/10.3390/ph15020186 |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a GBV_NLM | ||
951 | |a AR | ||
952 | |d 15 |j 2022 |e 2 |b 02 |c 02 |