Antioxidant-biocompatible and stable catalase-based gelatin-alginate hydrogel scaffold with thermal wound healing capability : immobilization and delivery approach
© The Author(s) 2022..
Hydrogel-based matrix prepared using biopolymers is a new frontier of emerging platforms for enzyme immobilization for biomedical applications. Catalase (CAT) delivery can be effective in inhibiting reactive oxygen species (ROS)-mediated prolongation of the wound healing process. In this study, to improve CAT stability for effective application, gelatin(Gel)-alginate (Alg) biocompatible hydrogel (Gel-Alg), as immobilization support, was prepared using calcium chloride as an ionic cross-linker. High entrapment efficiency of 92% was obtained with 2% Gel and 1.5% Alg. Hydrogel immobilized CAT (CAT-Gel-Alg) showed a wide range of pH from 4 to 9 and temperature stability between 20 to 60 °C, compared to free CAT. CAT-Gel-Alg kinetic parameters revealed an increased K m (24.15 mM) and a decreased V max (1.39 µmol H2O2/mg protein min) × 104. CAT-Gel-Alg retained 52% of its original activity after 20 consecutive catalytic runs and displayed improved thermal stability with a higher t 1/2 value (half-life of 100.43 vs. 46 min). In addition, 85% of the initial activity was maintained after 8 weeks' storage at 4 °C. At 24 h after thermal injury, a statistically significant difference in lesion sizes between the treated group and the control group was reported. Finally, our findings suggest that the superior CAT-Gel-Alg stability and reusability are resonant features for efficient biomedical applications, and ROS scavenging by CAT in the post-burn phase offers protection for local treatment of burned tissues with encouraging wound healing kinetics.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
3 Biotech - 12(2022), 3 vom: 05. März, Seite 73 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Abdel-Mageed, Heidi Mohamed [VerfasserIn] |
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| Links: |
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| Themen: |
Burn wound healing |
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| Anmerkungen: |
Date Revised 17.03.2022 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1007/s13205-022-03131-4 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM33742909X |
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| 245 | 1 | 0 | |a Antioxidant-biocompatible and stable catalase-based gelatin-alginate hydrogel scaffold with thermal wound healing capability |b immobilization and delivery approach |
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| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © The Author(s) 2022. | ||
| 520 | |a Hydrogel-based matrix prepared using biopolymers is a new frontier of emerging platforms for enzyme immobilization for biomedical applications. Catalase (CAT) delivery can be effective in inhibiting reactive oxygen species (ROS)-mediated prolongation of the wound healing process. In this study, to improve CAT stability for effective application, gelatin(Gel)-alginate (Alg) biocompatible hydrogel (Gel-Alg), as immobilization support, was prepared using calcium chloride as an ionic cross-linker. High entrapment efficiency of 92% was obtained with 2% Gel and 1.5% Alg. Hydrogel immobilized CAT (CAT-Gel-Alg) showed a wide range of pH from 4 to 9 and temperature stability between 20 to 60 °C, compared to free CAT. CAT-Gel-Alg kinetic parameters revealed an increased K m (24.15 mM) and a decreased V max (1.39 µmol H2O2/mg protein min) × 104. CAT-Gel-Alg retained 52% of its original activity after 20 consecutive catalytic runs and displayed improved thermal stability with a higher t 1/2 value (half-life of 100.43 vs. 46 min). In addition, 85% of the initial activity was maintained after 8 weeks' storage at 4 °C. At 24 h after thermal injury, a statistically significant difference in lesion sizes between the treated group and the control group was reported. Finally, our findings suggest that the superior CAT-Gel-Alg stability and reusability are resonant features for efficient biomedical applications, and ROS scavenging by CAT in the post-burn phase offers protection for local treatment of burned tissues with encouraging wound healing kinetics | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Burn wound healing | |
| 650 | 4 | |a Catalase immobilization | |
| 650 | 4 | |a Gelatin alginate biopolymers | |
| 650 | 4 | |a Half-life | |
| 650 | 4 | |a Hydrogel | |
| 650 | 4 | |a Stabilization | |
| 650 | 4 | |a Thermal injury | |
| 700 | 1 | |a Abd El Aziz, Amira Emad |e verfasserin |4 aut | |
| 700 | 1 | |a Abdel Raouf, Batoul Mohamed |e verfasserin |4 aut | |
| 700 | 1 | |a Mohamed, Saleh Ahmed |e verfasserin |4 aut | |
| 700 | 1 | |a Nada, Dina |e verfasserin |4 aut | |
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