~1H-qNMR quantification of total ginsenosides in Shenmai Injection
A content determination method based on ~1H-qNMR was developed for the determination of total ginsenosides in Shenmai Injection. The parameters were optimized with CD_3OD as the solvent, dimethyl terephthalate as the internal standard, the peak at δ 8.11 as the internal standard peak, and the peaks at δ 1.68 and δ 0.79 as quantitative peaks of total ginsenosides. The developed ~1H-qNMR-based method was validated methodologically. The results showed that the method could achieve accurate measurement of total ginsenosides in Shenmai Injection in the range of 0.167 6-3.091 1 mmol·L~(-1). The developed ~1H-qNMR-based method for total ginsenosides is simple in operation, short in analysis time, strong in specificity, independent of accompanying standard curve, and small in sample volume, which can serve as a reliable mean for the quality control of Shenmai Injection. This study is expected to provide new ideas for the development of quantification methods of total ginsenosides.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:47 |
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| Enthalten in: |
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 47(2022), 3 vom: 14. Feb., Seite 587-592 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Li, Wen-Zhu [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 21.02.2022 Date Revised 21.02.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.19540/j.cnki.cjcmm.20211108.302 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM337108218 |
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| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a A content determination method based on ~1H-qNMR was developed for the determination of total ginsenosides in Shenmai Injection. The parameters were optimized with CD_3OD as the solvent, dimethyl terephthalate as the internal standard, the peak at δ 8.11 as the internal standard peak, and the peaks at δ 1.68 and δ 0.79 as quantitative peaks of total ginsenosides. The developed ~1H-qNMR-based method was validated methodologically. The results showed that the method could achieve accurate measurement of total ginsenosides in Shenmai Injection in the range of 0.167 6-3.091 1 mmol·L~(-1). The developed ~1H-qNMR-based method for total ginsenosides is simple in operation, short in analysis time, strong in specificity, independent of accompanying standard curve, and small in sample volume, which can serve as a reliable mean for the quality control of Shenmai Injection. This study is expected to provide new ideas for the development of quantification methods of total ginsenosides | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Shenmai Injection | |
| 650 | 4 | |a nuclear magnetic resonance | |
| 650 | 4 | |a quantification method | |
| 650 | 4 | |a total ginsenosides | |
| 650 | 4 | |a ~1H-qNMR | |
| 650 | 7 | |a Drug Combinations |2 NLM | |
| 650 | 7 | |a Drugs, Chinese Herbal |2 NLM | |
| 650 | 7 | |a Ginsenosides |2 NLM | |
| 650 | 7 | |a fructus schizandrae, radix ginseng, radix ophiopogonis drug combination |2 NLM | |
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| 700 | 1 | |a Zhao, Fang |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Jian-Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Qu, Hai-Bin |e verfasserin |4 aut | |
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