Mechanism of spindle pole organization and instability in human oocytes
Human oocytes are prone to assembling meiotic spindles with unstable poles, which can favor aneuploidy in human eggs. The underlying causes of spindle instability are unknown. We found that NUMA (nuclear mitotic apparatus protein)-mediated clustering of microtubule minus ends focused the spindle poles in human, bovine, and porcine oocytes and in mouse oocytes depleted of acentriolar microtubule-organizing centers (aMTOCs). However, unlike human oocytes, bovine, porcine, and aMTOC-free mouse oocytes have stable spindles. We identified the molecular motor KIFC1 (kinesin superfamily protein C1) as a spindle-stabilizing protein that is deficient in human oocytes. Depletion of KIFC1 recapitulated spindle instability in bovine and aMTOC-free mouse oocytes, and the introduction of exogenous KIFC1 rescued spindle instability in human oocytes. Thus, the deficiency of KIFC1 contributes to spindle instability in human oocytes.
| Errataetall: |
CommentIn: J Assist Reprod Genet. 2022 Mar;39(3):553-554. - PMID 35348951 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:375 |
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| Enthalten in: |
Science (New York, N.Y.) - 375(2022), 6581 vom: 11. Feb., Seite eabj3944 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
So, Chun [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 18.02.2022 Date Revised 25.04.2022 published: Print-Electronic CommentIn: J Assist Reprod Genet. 2022 Mar;39(3):553-554. - PMID 35348951 Citation Status MEDLINE |
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| doi: |
10.1126/science.abj3944 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Mechanism of spindle pole organization and instability in human oocytes |
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| 500 | |a CommentIn: J Assist Reprod Genet. 2022 Mar;39(3):553-554. - PMID 35348951 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Human oocytes are prone to assembling meiotic spindles with unstable poles, which can favor aneuploidy in human eggs. The underlying causes of spindle instability are unknown. We found that NUMA (nuclear mitotic apparatus protein)-mediated clustering of microtubule minus ends focused the spindle poles in human, bovine, and porcine oocytes and in mouse oocytes depleted of acentriolar microtubule-organizing centers (aMTOCs). However, unlike human oocytes, bovine, porcine, and aMTOC-free mouse oocytes have stable spindles. We identified the molecular motor KIFC1 (kinesin superfamily protein C1) as a spindle-stabilizing protein that is deficient in human oocytes. Depletion of KIFC1 recapitulated spindle instability in bovine and aMTOC-free mouse oocytes, and the introduction of exogenous KIFC1 rescued spindle instability in human oocytes. Thus, the deficiency of KIFC1 contributes to spindle instability in human oocytes | ||
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| 700 | 1 | |a Lukinavičius, Gražvydas |e verfasserin |4 aut | |
| 700 | 1 | |a Möbius, Wiebke |e verfasserin |4 aut | |
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