MicroRNA-101a-3p mimic ameliorates spinal cord ischemia/reperfusion injury

miR-101a-3p is expressed in a variety of organs and tissues and plays a regulatory role in many diseases, but its role in spinal cord ischemia/reperfusion injury remains unclear. In this study, we established a rat model of spinal cord ischemia/reperfusion injury by clamping the aortic arch for 14 minutes followed by reperfusion for 24 hours. Results showed that miR-101a-3p expression in L4-L6 spinal cord was greatly decreased, whereas MYCN expression was greatly increased. Dual-luciferase reporter assay results showed that miR-101a-3p targeted MYCN. MYCN immunoreactivity, which was primarily colocalized with neurons in L4-L6 spinal tissue, greatly increased after spinal cord ischemia/reperfusion injury. However, intrathecal injection of an miR-101a-3p mimic within 24 hours before injury decreased MYCN, p53, caspase-9 and interleukin-1β expression, reduced p53 immunoreactivity, reduced the number of MYCN/NeuN-positive cells and the number of necrotic cells in L4-L6 spinal tissue, and increased Tarlov scores. These findings suggest that the miR-101a-3p mimic improved spinal ischemia/reperfusion injury-induced nerve cell apoptosis and inflammation by inhibiting MYCN and the p53 signaling pathway. Therefore, miR-101a-3p mimic therapy may be a potential treatment option for spinal ischemia/reperfusion injury.

Medienart:

E-Artikel

Erscheinungsjahr:

2022

Erschienen:

2022

Enthalten in:

Zur Gesamtaufnahme - volume:17

Enthalten in:

Neural regeneration research - 17(2022), 9 vom: 08. Sept., Seite 2022-2028

Sprache:

Englisch

Beteiligte Personen:

Zhang, Zai-Li [VerfasserIn]
Wang, Dan [VerfasserIn]
Chen, Feng-Shou [VerfasserIn]

Links:

Volltext

Themen:

Apoptosis
Caspase-9
Inflammation
Interleukin-1βmicroRNA-101a-3p
Journal Article
MYCN
Nerve cells
P53
Spinal cord ischemia/reperfusion injury

Anmerkungen:

Date Revised 09.03.2022

published: Print

Citation Status PubMed-not-MEDLINE

doi:

10.4103/1673-5374.335164

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM336749260