Enrichment of a denitratating microbial community through kinetic limitation
Published by Elsevier Ltd..
Denitratation, or the intentionally engineered accumulation of nitrite (NO2-) from selective reduction of nitrate (NO3-), can be combined with downstream anammox to reduce chemical and energy use associated with conventional nitrification and denitrification. This study aimed to enrich a denitratating microbial community capable of significant NO2- accumulation by applying added kinetic limitation to an already stoichiometrically-limited, glycerol-driven denitratation process. Operation at solids residence time, SRT=3.0 d, resulted in optimal denitratation performance and a microbial community dominated by NO3--respirers, noted by one order of magnitude lower total copy numbers of nirS and nirK gene transcripts compared to longer SRTs. Selective NO3- reduction to NO2- was achieved at all SRTs although longer SRTs (less kinetic limitation) supported microbial communities more capable of full denitrification as described by a lower NO2- accumulation ratio (NAR=42±5%) and higher steady-state nitrous oxide (1.5 mg/L N2O-N) accumulation. Shorter SRTs (more kinetic limitation) led to higher observed yields (Y=0.63 mg-COD/mg-COD) with more electrons dedicated for cell synthesis (fs=0.56±0.10), which potentially contributed to the accumulation of NO3-. Enrichment of a denitratating-dominant microbial community by optimizing kinetic limitation operating parameters could support significant NO2- accumulation and reduce chemical and energy use for biological nitrogen removal when combined with downstream anammox.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:161 |
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| Enthalten in: |
Environment international - 161(2022) vom: 15. März, Seite 107113 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Baideme, Matthew [VerfasserIn] |
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| Links: |
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| Themen: |
Denitratation |
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| Anmerkungen: |
Date Completed 16.03.2022 Date Revised 16.03.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.envint.2022.107113 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Denitratation, or the intentionally engineered accumulation of nitrite (NO2-) from selective reduction of nitrate (NO3-), can be combined with downstream anammox to reduce chemical and energy use associated with conventional nitrification and denitrification. This study aimed to enrich a denitratating microbial community capable of significant NO2- accumulation by applying added kinetic limitation to an already stoichiometrically-limited, glycerol-driven denitratation process. Operation at solids residence time, SRT=3.0 d, resulted in optimal denitratation performance and a microbial community dominated by NO3--respirers, noted by one order of magnitude lower total copy numbers of nirS and nirK gene transcripts compared to longer SRTs. Selective NO3- reduction to NO2- was achieved at all SRTs although longer SRTs (less kinetic limitation) supported microbial communities more capable of full denitrification as described by a lower NO2- accumulation ratio (NAR=42±5%) and higher steady-state nitrous oxide (1.5 mg/L N2O-N) accumulation. Shorter SRTs (more kinetic limitation) led to higher observed yields (Y=0.63 mg-COD/mg-COD) with more electrons dedicated for cell synthesis (fs=0.56±0.10), which potentially contributed to the accumulation of NO3-. Enrichment of a denitratating-dominant microbial community by optimizing kinetic limitation operating parameters could support significant NO2- accumulation and reduce chemical and energy use for biological nitrogen removal when combined with downstream anammox | ||
| 650 | 4 | |a Journal Article | |
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