Microbiological diagnosis of implant-associated infections : Retrospective analysis of 133 patients in an arthroplasty center
© 2022. The Author(s), under exclusive licence to Springer Medizin Verlag GmbH, ein Teil von Springer Nature..
BACKGROUND: Because standardized microbiological cultures of puncture fluids and tissue samples often do not provide pathogen detection in implant-associated infections, sonication and polymerase chain reaction (PCR) are used additionally today.
OBJECTIVES: Pathogen spectra and previous microbiological standards are examined for agreement of results using the new methods sonication and PCR.
MATERIALS AND METHODS: In this descriptive, retrospective observational study, we evaluated the data of 133 patients in whom a joint prosthesis, osteosynthesis material or a spacer was removed during revision surgery with suspected implant-associated infection and sent for sonication.
RESULTS: Pathogen detection was achieved by culture of peri-implant material in 40.1% and by sonication in 42.5%. In each case, coagulase-negative staphylococci were detected most frequently. Overall, the results were consistent in 71.7% of cases. In the discrepant cases, more anaerobes could be detected by sonication, especially for osteosynthesis material and knee prostheses. PCR analyses in 21 cases showed pathogen detection in 14.3% and agreement with the results of peri-implant tissue culture and sonication in 57.1% and 66.7%, respectively.
CONCLUSIONS: The present results indicate a gain in sensitivity of sonication, especially for anaerobes that are difficult to grow, and a gain in specificity through sonication. PCR analyses should be reserved for specific questions.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:51 |
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| Enthalten in: |
Orthopadie (Heidelberg, Germany) - 51(2022), 7 vom: 07. Juli, Seite 531-539 |
| Sprache: |
Deutsch |
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| Weiterer Titel: |
Mikrobiologische Diagnostik bei implantatassoziierten Infektionen : Retrospektive Analyse von 133 Patienten in einem Endoprothetikzentrum |
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| Beteiligte Personen: |
Spickermann, J [VerfasserIn] |
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| Links: |
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| Themen: |
Biofilm |
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| Anmerkungen: |
Date Completed 06.07.2022 Date Revised 06.07.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s00132-022-04212-1 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a Microbiological diagnosis of implant-associated infections |b Retrospective analysis of 133 patients in an arthroplasty center |
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| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022. The Author(s), under exclusive licence to Springer Medizin Verlag GmbH, ein Teil von Springer Nature. | ||
| 520 | |a BACKGROUND: Because standardized microbiological cultures of puncture fluids and tissue samples often do not provide pathogen detection in implant-associated infections, sonication and polymerase chain reaction (PCR) are used additionally today | ||
| 520 | |a OBJECTIVES: Pathogen spectra and previous microbiological standards are examined for agreement of results using the new methods sonication and PCR | ||
| 520 | |a MATERIALS AND METHODS: In this descriptive, retrospective observational study, we evaluated the data of 133 patients in whom a joint prosthesis, osteosynthesis material or a spacer was removed during revision surgery with suspected implant-associated infection and sent for sonication | ||
| 520 | |a RESULTS: Pathogen detection was achieved by culture of peri-implant material in 40.1% and by sonication in 42.5%. In each case, coagulase-negative staphylococci were detected most frequently. Overall, the results were consistent in 71.7% of cases. In the discrepant cases, more anaerobes could be detected by sonication, especially for osteosynthesis material and knee prostheses. PCR analyses in 21 cases showed pathogen detection in 14.3% and agreement with the results of peri-implant tissue culture and sonication in 57.1% and 66.7%, respectively | ||
| 520 | |a CONCLUSIONS: The present results indicate a gain in sensitivity of sonication, especially for anaerobes that are difficult to grow, and a gain in specificity through sonication. PCR analyses should be reserved for specific questions | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Observational Study | |
| 650 | 4 | |a Biofilm | |
| 650 | 4 | |a Pathogen distribution | |
| 650 | 4 | |a Periprosthetic joint infection | |
| 650 | 4 | |a Polymerase chain reaction | |
| 650 | 4 | |a Sonication | |
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| 700 | 1 | |a Steinmann, J |e verfasserin |4 aut | |
| 700 | 1 | |a Ziegler, R |e verfasserin |4 aut | |
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