A molecular atlas of innate immunity to adjuvanted and live attenuated vaccines, in mice
© 2022. The Author(s)..
Adjuvants hold great potential in enhancing vaccine efficacy, making the understanding and improving of adjuvants critical goals in vaccinology. The TLR7/8 agonist, 3M-052, induces long-lived humoral immunity in non-human primates and is currently being evaluated in human clinical trials. However, the innate mechanisms of 3M-052 have not been fully characterized. Here, we perform flow cytometry, single cell RNA-seq and ATAC-seq to profile the kinetics, transcriptomics and epigenomics of innate immune cells in murine draining lymph nodes following 3M-052-Alum/Ovalbumin immunization. We find that 3M-052-Alum/OVA induces a robust antiviral and interferon gene program, similar to the yellow fever vaccine, which is known to confer long-lasting protection. Activation of myeloid cells in dLNs persists through day 28 and single cell analysis reveals putative TF-gene regulatory programs in distinct myeloid cells and heterogeneity of monocytes. This study provides a comprehensive characterization of the transcriptomics and epigenomics of innate populations in the dLNs after vaccination.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:13 |
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| Enthalten in: |
Nature communications - 13(2022), 1 vom: 27. Jan., Seite 549 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lee, Audrey [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 17.02.2022 Date Revised 16.12.2022 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1038/s41467-022-28197-9 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM336214448 |
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| 100 | 1 | |a Lee, Audrey |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A molecular atlas of innate immunity to adjuvanted and live attenuated vaccines, in mice |
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| 500 | |a Date Revised 16.12.2022 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2022. The Author(s). | ||
| 520 | |a Adjuvants hold great potential in enhancing vaccine efficacy, making the understanding and improving of adjuvants critical goals in vaccinology. The TLR7/8 agonist, 3M-052, induces long-lived humoral immunity in non-human primates and is currently being evaluated in human clinical trials. However, the innate mechanisms of 3M-052 have not been fully characterized. Here, we perform flow cytometry, single cell RNA-seq and ATAC-seq to profile the kinetics, transcriptomics and epigenomics of innate immune cells in murine draining lymph nodes following 3M-052-Alum/Ovalbumin immunization. We find that 3M-052-Alum/OVA induces a robust antiviral and interferon gene program, similar to the yellow fever vaccine, which is known to confer long-lasting protection. Activation of myeloid cells in dLNs persists through day 28 and single cell analysis reveals putative TF-gene regulatory programs in distinct myeloid cells and heterogeneity of monocytes. This study provides a comprehensive characterization of the transcriptomics and epigenomics of innate populations in the dLNs after vaccination | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, N.I.H., Extramural | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Adjuvants, Immunologic |2 NLM | |
| 650 | 7 | |a Alum Compounds |2 NLM | |
| 650 | 7 | |a Antibodies, Viral |2 NLM | |
| 650 | 7 | |a Membrane Glycoproteins |2 NLM | |
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| 700 | 1 | |a Scott, Madeleine K D |e verfasserin |4 aut | |
| 700 | 1 | |a Wimmers, Florian |e verfasserin |4 aut | |
| 700 | 1 | |a Arunachalam, Prabhu S |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Wei |e verfasserin |4 aut | |
| 700 | 1 | |a Fox, Christopher B |e verfasserin |4 aut | |
| 700 | 1 | |a Tomai, Mark |e verfasserin |4 aut | |
| 700 | 1 | |a Khatri, Purvesh |e verfasserin |4 aut | |
| 700 | 1 | |a Pulendran, Bali |e verfasserin |4 aut | |
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