Kupffer Cell Hyaline Globules in Children With Autoimmune Hepatitis
© 2021 Indian National Association for Study of the Liver. Published by Elsevier B.V..
BACKGROUND: Hyaline globules (HGs) in the cytoplasm of Kupffer cells (KCs) have been appraised for being a typical feature of autoimmune hepatitis (AIH). This study aimed to determine how useful Kupffer cell hyaline globules (KCHGs) are in diagnosing AIH vs. other causes of pediatric chronic liver diseases (PCLDs).
MATERIALS AND METHODS: This retrospective study recruited 124 children; 58 with AIH, 50 with chronic hepatitis C virus (HCV) infection, and 16 with Wilson's disease (WD). Two pathologists retrieved paraffin blocks of liver biopsies and prepared new cut sections for Periodic acid-Schiff-Diastase (PAS-D) stain. They independently examined liver biopsies before starting treatment. Two pediatricians reviewed medical records for demographic, clinical, laboratory, and serological findings.
RESULTS: Females represented 48.6% of the studied children with a median age of 5.8 (4.9) years. Pathologists identified KCHGs in 67.24%, 12.5%, and 6.0% of AIH, WD, and HCV affected children respectively, P < 0.001. A significantly higher proportion of seropositive than seronegative AIH patients had KCHGs (77.5% vs. 50.0%), (P < 0.05). In multivariate analysis, KCHGs and prolonged prothrombin time were the only significant predictors that differentiate between AIH and the other studied PCLDs. The odds ratio of having AIH increased 68 times if KCHGs were seen. Among children with AIH, the presence of KCHGs was associated with higher median levels of direct bilirubin 2.2 (1.3) vs. 1.2 (2.2), and immunoglobulin G 3.2 (1.9) vs. 2.0 (1.7), (P < 0.05), but not to histopathological findings or hepatic fibrosis and activity.
CONCLUSIONS: KCHGs are key indicators that can differentiate between AIH and other PCLDs, and between seropositive and seronegative AIH.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:12 |
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| Enthalten in: |
Journal of clinical and experimental hepatology - 12(2022), 1 vom: 15. Jan., Seite 20-28 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Khedr, Mohammed A [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Revised 05.11.2023 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.jceh.2021.04.013 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM336033370 |
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| 100 | 1 | |a Khedr, Mohammed A |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Kupffer Cell Hyaline Globules in Children With Autoimmune Hepatitis |
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| 500 | |a Date Revised 05.11.2023 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2021 Indian National Association for Study of the Liver. Published by Elsevier B.V. | ||
| 520 | |a BACKGROUND: Hyaline globules (HGs) in the cytoplasm of Kupffer cells (KCs) have been appraised for being a typical feature of autoimmune hepatitis (AIH). This study aimed to determine how useful Kupffer cell hyaline globules (KCHGs) are in diagnosing AIH vs. other causes of pediatric chronic liver diseases (PCLDs) | ||
| 520 | |a MATERIALS AND METHODS: This retrospective study recruited 124 children; 58 with AIH, 50 with chronic hepatitis C virus (HCV) infection, and 16 with Wilson's disease (WD). Two pathologists retrieved paraffin blocks of liver biopsies and prepared new cut sections for Periodic acid-Schiff-Diastase (PAS-D) stain. They independently examined liver biopsies before starting treatment. Two pediatricians reviewed medical records for demographic, clinical, laboratory, and serological findings | ||
| 520 | |a RESULTS: Females represented 48.6% of the studied children with a median age of 5.8 (4.9) years. Pathologists identified KCHGs in 67.24%, 12.5%, and 6.0% of AIH, WD, and HCV affected children respectively, P < 0.001. A significantly higher proportion of seropositive than seronegative AIH patients had KCHGs (77.5% vs. 50.0%), (P < 0.05). In multivariate analysis, KCHGs and prolonged prothrombin time were the only significant predictors that differentiate between AIH and the other studied PCLDs. The odds ratio of having AIH increased 68 times if KCHGs were seen. Among children with AIH, the presence of KCHGs was associated with higher median levels of direct bilirubin 2.2 (1.3) vs. 1.2 (2.2), and immunoglobulin G 3.2 (1.9) vs. 2.0 (1.7), (P < 0.05), but not to histopathological findings or hepatic fibrosis and activity | ||
| 520 | |a CONCLUSIONS: KCHGs are key indicators that can differentiate between AIH and other PCLDs, and between seropositive and seronegative AIH | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a AIH, Autoimmune hepatitis | |
| 650 | 4 | |a ALP, Alkaline phosphatase | |
| 650 | 4 | |a ALT, Alanine aminotransferase | |
| 650 | 4 | |a AMA, Antimitochondrial antibody | |
| 650 | 4 | |a ANA, Antinuclear antibody | |
| 650 | 4 | |a AST, Aspartate aminotransferase | |
| 650 | 4 | |a GGT, Gamma-glutamyl transpeptidase | |
| 650 | 4 | |a H&E, Hematoxylin and eosin | |
| 650 | 4 | |a HCV, Hepatitis C virus | |
| 650 | 4 | |a IAIHG, International Autoimmune Hepatitis Group | |
| 650 | 4 | |a KCHGs, Kupffer cell hyaline globules | |
| 650 | 4 | |a KCs, Kupffer cells | |
| 650 | 4 | |a Kupffer cells | |
| 650 | 4 | |a LKMA, Liver-kidney microsome 1 antibody | |
| 650 | 4 | |a PAS, Periodic Acid Schiff | |
| 650 | 4 | |a PAS-D, Periodic acid–Schiff–Diastase | |
| 650 | 4 | |a PCLD, Pediatric chronic liver disease | |
| 650 | 4 | |a SMA, Smooth muscle antibody | |
| 650 | 4 | |a WBCs, White blood cell count | |
| 650 | 4 | |a WD, Wilson’s disease | |
| 650 | 4 | |a Wilson’s disease | |
| 650 | 4 | |a antiLC1, Antiliver cytosol type 1 antibody | |
| 650 | 4 | |a autoimmune hepatitis | |
| 650 | 4 | |a hepatitis C virus infection | |
| 650 | 4 | |a hyaline globules | |
| 650 | 4 | |a liver histopathology | |
| 700 | 1 | |a Adawy, Nermin M |e verfasserin |4 aut | |
| 700 | 1 | |a Salim, Tahany A |e verfasserin |4 aut | |
| 700 | 1 | |a Salem, Menan E |e verfasserin |4 aut | |
| 700 | 1 | |a Ghazy, Ramy M |e verfasserin |4 aut | |
| 700 | 1 | |a Elharoun, Ahmed S |e verfasserin |4 aut | |
| 700 | 1 | |a Sultan, Mervat M |e verfasserin |4 aut | |
| 700 | 1 | |a Ehsan, Nermine A |e verfasserin |4 aut | |
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