Comparison of Plaque Size, Thermal Stability, and Replication Rate among SARS-CoV-2 Variants of Concern
SARS-CoV-2, like other RNA viruses, has a propensity for genetic evolution owing to the low fidelity of its viral polymerase. Several recent reports have described a series of novel SARS-CoV-2 variants. Some of these have been identified as variants of concern (VOCs), including alpha (B.1.1.7, Clade GRY), beta (B.1.351, Clade GH), gamma (P.1, Clade GR), and delta (B.1.617.2, Clade G). VOCs are likely to have some effect on transmissibility, antibody evasion, and changes in therapeutic or vaccine effectiveness. However, the physiological and virological understanding of these variants remains poor. We demonstrated that these four VOCs exhibited differences in plaque size, thermal stability at physiological temperature, and replication rates. The mean plaque size of beta was the largest, followed by those of gamma, delta, and alpha. Thermal stability, evaluated by measuring infectivity and half-life after prolonged incubation at physiological temperature, was correlated with plaque size in all variants except alpha. However, despite its relatively high thermal stability, alpha's small plaque size resulted in lower replication rates and fewer progeny viruses. Our findings may inform further virological studies of SARS-CoV-2 variant characteristics, VOCs, and variants of interest. These studies are important for the effective management of the COVID-19 pandemic.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
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| Enthalten in: |
Viruses - 14(2021), 1 vom: 30. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Jeong, Gi Uk [VerfasserIn] |
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| Links: |
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| Themen: |
Comparative Study |
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| Anmerkungen: |
Date Completed 03.02.2022 Date Revised 03.02.2022 published: Electronic Citation Status MEDLINE |
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| doi: |
10.3390/v14010055 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a SARS-CoV-2, like other RNA viruses, has a propensity for genetic evolution owing to the low fidelity of its viral polymerase. Several recent reports have described a series of novel SARS-CoV-2 variants. Some of these have been identified as variants of concern (VOCs), including alpha (B.1.1.7, Clade GRY), beta (B.1.351, Clade GH), gamma (P.1, Clade GR), and delta (B.1.617.2, Clade G). VOCs are likely to have some effect on transmissibility, antibody evasion, and changes in therapeutic or vaccine effectiveness. However, the physiological and virological understanding of these variants remains poor. We demonstrated that these four VOCs exhibited differences in plaque size, thermal stability at physiological temperature, and replication rates. The mean plaque size of beta was the largest, followed by those of gamma, delta, and alpha. Thermal stability, evaluated by measuring infectivity and half-life after prolonged incubation at physiological temperature, was correlated with plaque size in all variants except alpha. However, despite its relatively high thermal stability, alpha's small plaque size resulted in lower replication rates and fewer progeny viruses. Our findings may inform further virological studies of SARS-CoV-2 variant characteristics, VOCs, and variants of interest. These studies are important for the effective management of the COVID-19 pandemic | ||
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| 700 | 1 | |a Moon, Hyun Woo |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Wooseong |e verfasserin |4 aut | |
| 700 | 1 | |a Hwang, Insu |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Haesoo |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Kyun-Do |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Chonsaeng |e verfasserin |4 aut | |
| 700 | 1 | |a Ahn, Dae-Gyun |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Bum-Tae |e verfasserin |4 aut | |
| 700 | 1 | |a Kim, Seong-Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Kwon, Young-Chan |e verfasserin |4 aut | |
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