TSS-seq of Toxoplasma gondii sporozoites revealed a novel motif in stage-specific promoters
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved..
Toxoplasma gondii is one of the most common zoonotic protozoan parasites. It has three major infectious stages: rapidly multiplying tachyzoites (Tz), slowly replicating bradyzoites (Bz) and a resting/free-living stage, sporozoites (Sz). The regulatory mechanisms governing stage-specific gene expression are not fully understood. Few transcriptional start sites (TSS) are known for Sz. In this study, we obtained TSS of Sz using an oligo-capping method and RNA-seq analysis. We identified 1,043,503 TSS in the Sz transcriptome. These defined 38,973 TSS clusters, of which, 11,925 were expressed in Sz and 1535 TSS differentially expressed in Sz. Based on these data, we defined promoter regions and novel sporozoite stage-specific motifs using MEME. TGTANNTACA was distributed around -55 to -75 regions from each TSS. Interestingly, the same motif was reported in another apicomplexan, Plasmodium berghei, as a cis-element of female-specific gametocyte genes, implying the presence of common regulatory machinery. Further comparative analysis should better define the distribution and function of these elements in other members of this important parasitic phylum.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:98 |
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| Enthalten in: |
Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 98(2022) vom: 01. März, Seite 105213 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Kidaka, Taishi [VerfasserIn] |
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| Links: |
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| Themen: |
Cis-elements |
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| Anmerkungen: |
Date Completed 04.03.2022 Date Revised 04.03.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.meegid.2022.105213 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM335766919 |
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| 245 | 1 | 0 | |a TSS-seq of Toxoplasma gondii sporozoites revealed a novel motif in stage-specific promoters |
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| 520 | |a Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved. | ||
| 520 | |a Toxoplasma gondii is one of the most common zoonotic protozoan parasites. It has three major infectious stages: rapidly multiplying tachyzoites (Tz), slowly replicating bradyzoites (Bz) and a resting/free-living stage, sporozoites (Sz). The regulatory mechanisms governing stage-specific gene expression are not fully understood. Few transcriptional start sites (TSS) are known for Sz. In this study, we obtained TSS of Sz using an oligo-capping method and RNA-seq analysis. We identified 1,043,503 TSS in the Sz transcriptome. These defined 38,973 TSS clusters, of which, 11,925 were expressed in Sz and 1535 TSS differentially expressed in Sz. Based on these data, we defined promoter regions and novel sporozoite stage-specific motifs using MEME. TGTANNTACA was distributed around -55 to -75 regions from each TSS. Interestingly, the same motif was reported in another apicomplexan, Plasmodium berghei, as a cis-element of female-specific gametocyte genes, implying the presence of common regulatory machinery. Further comparative analysis should better define the distribution and function of these elements in other members of this important parasitic phylum | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Sporozoites | |
| 650 | 4 | |a Toxoplasma gondii | |
| 650 | 4 | |a Transcriptional start sites | |
| 650 | 4 | |a Transcriptome | |
| 650 | 4 | |a cis-elements | |
| 700 | 1 | |a Sugi, Tatsuki |e verfasserin |4 aut | |
| 700 | 1 | |a Hayashida, Kyoko |e verfasserin |4 aut | |
| 700 | 1 | |a Suzuki, Yutaka |e verfasserin |4 aut | |
| 700 | 1 | |a Xuan, Xuenan |e verfasserin |4 aut | |
| 700 | 1 | |a Dubey, Jitender P |e verfasserin |4 aut | |
| 700 | 1 | |a Yamagishi, Junya |e verfasserin |4 aut | |
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