SHORT-ROOT paralogs mediate feedforward regulation of D-type cyclin to promote nodule formation in soybean
Copyright © 2022 the Author(s). Published by PNAS..
Nitrogen fixation in soybean takes place in root nodules that arise from de novo cell divisions in the root cortex. Although several early nodulin genes have been identified, the mechanism behind the stimulation of cortical cell division during nodulation has not been fully resolved. Here we provide evidence that two paralogs of soybean SHORT-ROOT (GmSHR) play vital roles in soybean nodulation. Expression of GmSHR4 and GmSHR5 (GmSHR4/5) is induced in cortical cells at the beginning of nodulation, when the first cell divisions occur. The expression level of GmSHR4/5 is positively associated with cortical cell division and nodulation. Knockdown of GmSHR5 inhibits cell division in outer cortical layers during nodulation. Knockdown of both paralogs disrupts the cell division throughout the cortex, resulting in poorly organized nodule primordia with delayed vascular tissue formation. GmSHR4/5 function by enhancing cytokinin signaling and activating early nodulin genes. Interestingly, D-type cyclins act downstream of GmSHR4/5, and GmSHR4/5 form a feedforward loop regulating D-type cyclins. Overexpression of D-type cyclins in soybean roots also enhanced nodulation. Collectively, we conclude that the GmSHR4/5-mediated pathway represents a vital module that triggers cytokinin signaling and activates D-type cyclins during nodulation in soybean.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:119 |
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| Enthalten in: |
Proceedings of the National Academy of Sciences of the United States of America - 119(2022), 3 vom: 18. Jan. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Wang, Chunhua [VerfasserIn] |
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| Links: |
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| Themen: |
Cell division |
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| Anmerkungen: |
Date Completed 22.02.2022 Date Revised 13.12.2023 published: Print Citation Status MEDLINE |
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| doi: |
10.1073/pnas.2108641119 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM335571247 |
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| 100 | 1 | |a Wang, Chunhua |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a SHORT-ROOT paralogs mediate feedforward regulation of D-type cyclin to promote nodule formation in soybean |
| 264 | 1 | |c 2022 | |
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| 500 | |a Date Completed 22.02.2022 | ||
| 500 | |a Date Revised 13.12.2023 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2022 the Author(s). Published by PNAS. | ||
| 520 | |a Nitrogen fixation in soybean takes place in root nodules that arise from de novo cell divisions in the root cortex. Although several early nodulin genes have been identified, the mechanism behind the stimulation of cortical cell division during nodulation has not been fully resolved. Here we provide evidence that two paralogs of soybean SHORT-ROOT (GmSHR) play vital roles in soybean nodulation. Expression of GmSHR4 and GmSHR5 (GmSHR4/5) is induced in cortical cells at the beginning of nodulation, when the first cell divisions occur. The expression level of GmSHR4/5 is positively associated with cortical cell division and nodulation. Knockdown of GmSHR5 inhibits cell division in outer cortical layers during nodulation. Knockdown of both paralogs disrupts the cell division throughout the cortex, resulting in poorly organized nodule primordia with delayed vascular tissue formation. GmSHR4/5 function by enhancing cytokinin signaling and activating early nodulin genes. Interestingly, D-type cyclins act downstream of GmSHR4/5, and GmSHR4/5 form a feedforward loop regulating D-type cyclins. Overexpression of D-type cyclins in soybean roots also enhanced nodulation. Collectively, we conclude that the GmSHR4/5-mediated pathway represents a vital module that triggers cytokinin signaling and activates D-type cyclins during nodulation in soybean | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a GmCYCD6;1 | |
| 650 | 4 | |a GmSHR4/5 | |
| 650 | 4 | |a cell division | |
| 650 | 4 | |a cytokinin | |
| 650 | 4 | |a nodule primordia | |
| 650 | 7 | |a Cyclins |2 NLM | |
| 650 | 7 | |a Cytokinins |2 NLM | |
| 650 | 7 | |a Membrane Proteins |2 NLM | |
| 650 | 7 | |a Plant Proteins |2 NLM | |
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| 700 | 1 | |a Li, Meng |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Nengsong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Haiyang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Pengxue |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Liling |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Mengyuan |e verfasserin |4 aut | |
| 700 | 1 | |a Bian, Xinxin |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Mengxue |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Shasha |e verfasserin |4 aut | |
| 700 | 1 | |a Niu, Xufang |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Mengyao |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xinxin |e verfasserin |4 aut | |
| 700 | 1 | |a Sang, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Wentao |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Ertao |e verfasserin |4 aut | |
| 700 | 1 | |a Gallagher, Kimberly L |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Shuang |e verfasserin |4 aut | |
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