An effective strategy for improving the specific activity and saccharification efficiency of cellulase by pre-incubation with phenolic acids
Copyright © 2021 Elsevier Ltd. All rights reserved..
This short communication analyzed the effects of lignin-derived phenolic acid compounds on cellulase. Vanillic acid, syringic acid, ferulic acid, and isovanillic acid improved cellulase specific activity and saccharification efficiency. In the enzymatic hydrolysis process, the promotion effect of phenolic acid was concentration-dependent. The effect of low concentration of phenolic acids (less than 5 mM) was negligible. After pre-incubating 1 g cellulase with 5 mmol phenolic acid, FPase-specific activity, CMCase-specific activity, and pNPGase-specific activity increased by 57.06%, 136.79%, and 110.61%, respectively. After digestion with pre-incubated cellulase, the saccharification efficiency of phosphoric acid-swollen cellulose increased by 45.13%. Pre-incubation with phenolic acid improved the saccharification efficiency of cellulase. It might be helpful to enhance the comprehensive utilization capacity of lignin-derived compounds.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:346 |
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| Enthalten in: |
Bioresource technology - 346(2022) vom: 01. Feb., Seite 126644 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ran, Qiuping [VerfasserIn] |
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| Links: |
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| Themen: |
9004-34-6 |
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| Anmerkungen: |
Date Completed 02.02.2022 Date Revised 02.02.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.biortech.2021.126644 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM335088872 |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 Elsevier Ltd. All rights reserved. | ||
| 520 | |a This short communication analyzed the effects of lignin-derived phenolic acid compounds on cellulase. Vanillic acid, syringic acid, ferulic acid, and isovanillic acid improved cellulase specific activity and saccharification efficiency. In the enzymatic hydrolysis process, the promotion effect of phenolic acid was concentration-dependent. The effect of low concentration of phenolic acids (less than 5 mM) was negligible. After pre-incubating 1 g cellulase with 5 mmol phenolic acid, FPase-specific activity, CMCase-specific activity, and pNPGase-specific activity increased by 57.06%, 136.79%, and 110.61%, respectively. After digestion with pre-incubated cellulase, the saccharification efficiency of phosphoric acid-swollen cellulose increased by 45.13%. Pre-incubation with phenolic acid improved the saccharification efficiency of cellulase. It might be helpful to enhance the comprehensive utilization capacity of lignin-derived compounds | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Cellulase | |
| 650 | 4 | |a Phenolic acid | |
| 650 | 4 | |a Pre-incubation | |
| 650 | 4 | |a Specific activity | |
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| 650 | 7 | |a Cellulose |2 NLM | |
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| 700 | 1 | |a Li, Huanan |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Jiashu |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Mengtian |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Ying |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Lingfang |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Zhengbing |e verfasserin |4 aut | |
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