ORAI1 regulates sustained cytosolic free calcium fluctuations during breast cancer cell apoptosis and apoptotic resistance via a STIM1 independent pathway
© 2021 Federation of American Societies for Experimental Biology..
Excessive rapid increases in cytosolic free Ca2+ have a clear association with the induction of cancer cell death. Whereas, characterizing the Ca2+ signaling events that occur during the progression of the apoptotic cascade over a period of hours or days, has not yet been possible. Now using genetically encoded Ca2+ indicators complemented with automated epifluorescence microscopy we have shown that staurosporine-induced apoptosis in MDA-MB-231 breast cancer cells was associated with delayed development of cytosolic free Ca2+ fluctuations, which were then maintained for 24 h. These cytosolic free Ca2+ fluctuations were dependent on the Ca2+ channel ORAI1. Silencing of ORAI1, but not its canonical activators STIM1 and STIM2, promoted apoptosis in this model. The pathway for this regulation implicates a mechanism previously associated with the migration of cancer cells involving ORAI1, the chaperone protein SigmaR1, and Ca2+ -activated K+ channels.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:36 |
|---|---|
| Enthalten in: |
FASEB journal : official publication of the Federation of American Societies for Experimental Biology - 36(2022), 1 vom: 21. Jan., Seite e22108 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Bassett, John J [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 11.01.2022 Date Revised 11.01.2022 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1096/fj.202002031RR |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM334756391 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM334756391 | ||
| 003 | DE-627 | ||
| 005 | 20231225224404.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1096/fj.202002031RR |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1115.xml |
| 035 | |a (DE-627)NLM334756391 | ||
| 035 | |a (NLM)34939697 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Bassett, John J |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a ORAI1 regulates sustained cytosolic free calcium fluctuations during breast cancer cell apoptosis and apoptotic resistance via a STIM1 independent pathway |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 11.01.2022 | ||
| 500 | |a Date Revised 11.01.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 Federation of American Societies for Experimental Biology. | ||
| 520 | |a Excessive rapid increases in cytosolic free Ca2+ have a clear association with the induction of cancer cell death. Whereas, characterizing the Ca2+ signaling events that occur during the progression of the apoptotic cascade over a period of hours or days, has not yet been possible. Now using genetically encoded Ca2+ indicators complemented with automated epifluorescence microscopy we have shown that staurosporine-induced apoptosis in MDA-MB-231 breast cancer cells was associated with delayed development of cytosolic free Ca2+ fluctuations, which were then maintained for 24 h. These cytosolic free Ca2+ fluctuations were dependent on the Ca2+ channel ORAI1. Silencing of ORAI1, but not its canonical activators STIM1 and STIM2, promoted apoptosis in this model. The pathway for this regulation implicates a mechanism previously associated with the migration of cancer cells involving ORAI1, the chaperone protein SigmaR1, and Ca2+ -activated K+ channels | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a GCaMP6 | |
| 650 | 4 | |a ORAI1 | |
| 650 | 4 | |a breast cancer | |
| 650 | 4 | |a calcium signaling | |
| 650 | 4 | |a cell death | |
| 650 | 7 | |a Neoplasm Proteins |2 NLM | |
| 650 | 7 | |a ORAI1 Protein |2 NLM | |
| 650 | 7 | |a ORAI1 protein, human |2 NLM | |
| 650 | 7 | |a STIM1 protein, human |2 NLM | |
| 650 | 7 | |a Stromal Interaction Molecule 1 |2 NLM | |
| 650 | 7 | |a Calcium |2 NLM | |
| 650 | 7 | |a SY7Q814VUP |2 NLM | |
| 700 | 1 | |a Robitaille, Mélanie |e verfasserin |4 aut | |
| 700 | 1 | |a Peters, Amelia A |e verfasserin |4 aut | |
| 700 | 1 | |a Bong, Alice H L |e verfasserin |4 aut | |
| 700 | 1 | |a Taing, Meng-Wong |e verfasserin |4 aut | |
| 700 | 1 | |a Wood, Ian A |e verfasserin |4 aut | |
| 700 | 1 | |a Sadras, Francisco |e verfasserin |4 aut | |
| 700 | 1 | |a Roberts-Thomson, Sarah J |e verfasserin |4 aut | |
| 700 | 1 | |a Monteith, Gregory R |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t FASEB journal : official publication of the Federation of American Societies for Experimental Biology |d 1989 |g 36(2022), 1 vom: 21. Jan., Seite e22108 |w (DE-627)NLM01261730X |x 1530-6860 |7 nnns |
| 773 | 1 | 8 | |g volume:36 |g year:2022 |g number:1 |g day:21 |g month:01 |g pages:e22108 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1096/fj.202002031RR |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 36 |j 2022 |e 1 |b 21 |c 01 |h e22108 | ||