Species-selective targeting of pathogens revealed by the atypical structure and active site of Trypanosoma cruzi histone deacetylase DAC2
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved..
Writing and erasing of posttranslational modifications are crucial to phenotypic plasticity and antigenic variation of eukaryotic pathogens. Targeting pathogens' modification machineries, thus, represents a valid approach to fighting parasitic diseases. However, identification of parasitic targets and the development of selective anti-parasitic drugs still represent major bottlenecks. Here, we show that the zinc-dependent histone deacetylases (HDACs) of the protozoan parasite Trypanosoma cruzi are key regulators that have significantly diverged from their human counterparts. Depletion of T. cruzi class I HDACs tcDAC1 and tcDAC2 compromises cell-cycle progression and division, leading to cell death. Notably, tcDAC2 displays a deacetylase activity essential to the parasite and shows major structural differences with human HDACs. Specifically, tcDAC2 harbors a modular active site with a unique subpocket targeted by inhibitors showing substantial anti-parasitic effects in cellulo and in vivo. Thus, the targeting of the many atypical HDACs in pathogens can enable anti-parasitic selective chemical impairment.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
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| Enthalten in: |
Cell reports - 37(2021), 12 vom: 21. Dez., Seite 110129 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Marek, Martin [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 15.02.2022 Date Revised 15.02.2022 published: Print Citation Status MEDLINE |
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| doi: |
10.1016/j.celrep.2021.110129 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM334728266 |
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| 100 | 1 | |a Marek, Martin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Species-selective targeting of pathogens revealed by the atypical structure and active site of Trypanosoma cruzi histone deacetylase DAC2 |
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| 500 | |a Date Completed 15.02.2022 | ||
| 500 | |a Date Revised 15.02.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved. | ||
| 520 | |a Writing and erasing of posttranslational modifications are crucial to phenotypic plasticity and antigenic variation of eukaryotic pathogens. Targeting pathogens' modification machineries, thus, represents a valid approach to fighting parasitic diseases. However, identification of parasitic targets and the development of selective anti-parasitic drugs still represent major bottlenecks. Here, we show that the zinc-dependent histone deacetylases (HDACs) of the protozoan parasite Trypanosoma cruzi are key regulators that have significantly diverged from their human counterparts. Depletion of T. cruzi class I HDACs tcDAC1 and tcDAC2 compromises cell-cycle progression and division, leading to cell death. Notably, tcDAC2 displays a deacetylase activity essential to the parasite and shows major structural differences with human HDACs. Specifically, tcDAC2 harbors a modular active site with a unique subpocket targeted by inhibitors showing substantial anti-parasitic effects in cellulo and in vivo. Thus, the targeting of the many atypical HDACs in pathogens can enable anti-parasitic selective chemical impairment | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Trypanosoma cruzi | |
| 650 | 4 | |a atypical three-dimensional structure | |
| 650 | 4 | |a chemical inhibition | |
| 650 | 4 | |a eukaryotic parasites | |
| 650 | 4 | |a functional essentiality | |
| 650 | 4 | |a histone deacetylases | |
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| 650 | 7 | |a Histone Deacetylase Inhibitors |2 NLM | |
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| 700 | 1 | |a Ramos-Morales, Elizabeth |e verfasserin |4 aut | |
| 700 | 1 | |a Picchi-Constante, Gisele F A |e verfasserin |4 aut | |
| 700 | 1 | |a Bayer, Theresa |e verfasserin |4 aut | |
| 700 | 1 | |a Norström, Carina |e verfasserin |4 aut | |
| 700 | 1 | |a Herp, Daniel |e verfasserin |4 aut | |
| 700 | 1 | |a Sales-Junior, Policarpo A |e verfasserin |4 aut | |
| 700 | 1 | |a Guerra-Slompo, Eloise P |e verfasserin |4 aut | |
| 700 | 1 | |a Hausmann, Kristin |e verfasserin |4 aut | |
| 700 | 1 | |a Chakrabarti, Alokta |e verfasserin |4 aut | |
| 700 | 1 | |a Shaik, Tajith B |e verfasserin |4 aut | |
| 700 | 1 | |a Merz, Annika |e verfasserin |4 aut | |
| 700 | 1 | |a Troesch, Edouard |e verfasserin |4 aut | |
| 700 | 1 | |a Schmidtkunz, Karin |e verfasserin |4 aut | |
| 700 | 1 | |a Goldenberg, Samuel |e verfasserin |4 aut | |
| 700 | 1 | |a Pierce, Raymond J |e verfasserin |4 aut | |
| 700 | 1 | |a Mourão, Marina M |e verfasserin |4 aut | |
| 700 | 1 | |a Jung, Manfred |e verfasserin |4 aut | |
| 700 | 1 | |a Schultz, Johan |e verfasserin |4 aut | |
| 700 | 1 | |a Sippl, Wolfgang |e verfasserin |4 aut | |
| 700 | 1 | |a Zanchin, Nilson I T |e verfasserin |4 aut | |
| 700 | 1 | |a Romier, Christophe |e verfasserin |4 aut | |
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