Combined berberine and probiotic treatment as an effective regimen for improving postprandial hyperlipidemia in type 2 diabetes patients : a double blinded placebo controlled randomized study
Non-fasting lipidemia (nFL), mainly contributed by postprandial lipidemia (PL), has recently been recognized as an important cardiovascular disease (CVD) risk as fasting lipidemia (FL). PL serves as a common feature of dyslipidemia in Type 2 Diabetes (T2D), albeit effective therapies targeting on PL were limited. In this study, we aimed to evaluate whether the therapy combining probiotics (Prob) and berberine (BBR), a proven antidiabetic and hypolipidemic regimen via altering gut microbiome, could effectively reduce PL in T2D and to explore the underlying mechanism. Blood PL (120 min after taking 100 g standard carbohydrate meal) was examined in 365 participants with T2D from the Probiotics and BBR on the Efficacy and Change of Gut Microbiota in Patients with Newly Diagnosed Type 2 Diabetes (PREMOTE study), a random, placebo-controlled, and multicenter clinical trial. Prob+BBR was superior to BBR or Prob alone in improving postprandial total cholesterol (pTC) and low-density lipoprotein cholesterol (pLDLc) levels with decrement of multiple species of postprandial lipidomic metabolites after 3 months follow-up. This effect was linked to the changes of fecal Bifidobacterium breve level responding to BBR alone or Prob+BBR treatment. Four fadD genes encoding long-chain acyl-CoA synthetase were identified in the genome of this B. breve strain, and transcriptionally activated by BBR. In vitro BBR treatment further decreased the concentration of FFA in the culture medium of B. breve compared to vehicle. Thus, the activation of fadD by BBR could enhance FFA import and mobilization in B. breve and diliminish the intraluminal lipids for absorption to mediate the effect of Prob+BBR on PL. Our study confirmed that BBR and Prob (B. breve) could exert a synergistic hypolipidemic effect on PL, acting as a gut lipid sink to achieve better lipidemia and CVD risk control in T2D.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:14 |
|---|---|
| Enthalten in: |
Gut microbes - 14(2022), 1 vom: 31. Jan., Seite 2003176 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Shujie [VerfasserIn] |
|---|
| Links: |
|---|
| Anmerkungen: |
Date Completed 24.02.2022 Date Revised 24.02.2022 published: Print Citation Status MEDLINE |
|---|
| doi: |
10.1080/19490976.2021.2003176 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM334599776 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM334599776 | ||
| 003 | DE-627 | ||
| 005 | 20231225224043.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1080/19490976.2021.2003176 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1115.xml |
| 035 | |a (DE-627)NLM334599776 | ||
| 035 | |a (NLM)34923903 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Shujie |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Combined berberine and probiotic treatment as an effective regimen for improving postprandial hyperlipidemia in type 2 diabetes patients |b a double blinded placebo controlled randomized study |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 24.02.2022 | ||
| 500 | |a Date Revised 24.02.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Non-fasting lipidemia (nFL), mainly contributed by postprandial lipidemia (PL), has recently been recognized as an important cardiovascular disease (CVD) risk as fasting lipidemia (FL). PL serves as a common feature of dyslipidemia in Type 2 Diabetes (T2D), albeit effective therapies targeting on PL were limited. In this study, we aimed to evaluate whether the therapy combining probiotics (Prob) and berberine (BBR), a proven antidiabetic and hypolipidemic regimen via altering gut microbiome, could effectively reduce PL in T2D and to explore the underlying mechanism. Blood PL (120 min after taking 100 g standard carbohydrate meal) was examined in 365 participants with T2D from the Probiotics and BBR on the Efficacy and Change of Gut Microbiota in Patients with Newly Diagnosed Type 2 Diabetes (PREMOTE study), a random, placebo-controlled, and multicenter clinical trial. Prob+BBR was superior to BBR or Prob alone in improving postprandial total cholesterol (pTC) and low-density lipoprotein cholesterol (pLDLc) levels with decrement of multiple species of postprandial lipidomic metabolites after 3 months follow-up. This effect was linked to the changes of fecal Bifidobacterium breve level responding to BBR alone or Prob+BBR treatment. Four fadD genes encoding long-chain acyl-CoA synthetase were identified in the genome of this B. breve strain, and transcriptionally activated by BBR. In vitro BBR treatment further decreased the concentration of FFA in the culture medium of B. breve compared to vehicle. Thus, the activation of fadD by BBR could enhance FFA import and mobilization in B. breve and diliminish the intraluminal lipids for absorption to mediate the effect of Prob+BBR on PL. Our study confirmed that BBR and Prob (B. breve) could exert a synergistic hypolipidemic effect on PL, acting as a gut lipid sink to achieve better lipidemia and CVD risk control in T2D | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Randomized Controlled Trial | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Type 2 diabetes | |
| 650 | 4 | |a berberine | |
| 650 | 4 | |a dyslipidemia | |
| 650 | 4 | |a gut microbiome | |
| 650 | 4 | |a postprandial lipidemia | |
| 650 | 4 | |a probiotics | |
| 650 | 7 | |a Cholesterol, LDL |2 NLM | |
| 650 | 7 | |a Berberine |2 NLM | |
| 650 | 7 | |a 0I8Y3P32UF |2 NLM | |
| 650 | 7 | |a Cholesterol |2 NLM | |
| 650 | 7 | |a 97C5T2UQ7J |2 NLM | |
| 700 | 1 | |a Ren, Huahui |e verfasserin |4 aut | |
| 700 | 1 | |a Zhong, Huanzi |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Xinjie |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Changkun |e verfasserin |4 aut | |
| 700 | 1 | |a Ma, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Xuejiang |e verfasserin |4 aut | |
| 700 | 1 | |a Xue, Yaoming |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Shan |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Jialin |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Gang |e verfasserin |4 aut | |
| 700 | 1 | |a Qu, Shen |e verfasserin |4 aut | |
| 700 | 1 | |a Liang, Jun |e verfasserin |4 aut | |
| 700 | 1 | |a Qin, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Qin |e verfasserin |4 aut | |
| 700 | 1 | |a Peng, Yongde |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xiaolin |e verfasserin |4 aut | |
| 700 | 1 | |a Zou, Yuanqiang |e verfasserin |4 aut | |
| 700 | 1 | |a Shi, Zhun |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xuelin |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Tingting |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Huanming |e verfasserin |4 aut | |
| 700 | 1 | |a Lai, Shenghan |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Guowang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Junhua |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Yifei |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Yanyun |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Weiqing |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Gut microbes |d 2010 |g 14(2022), 1 vom: 31. Jan., Seite 2003176 |w (DE-627)NLM199892369 |x 1949-0984 |7 nnns |
| 773 | 1 | 8 | |g volume:14 |g year:2022 |g number:1 |g day:31 |g month:01 |g pages:2003176 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1080/19490976.2021.2003176 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 14 |j 2022 |e 1 |b 31 |c 01 |h 2003176 | ||