A one-step reverse transcription recombinase polymerase amplification assay for lateral flow-based visual detection of PVY
Copyright © 2021 Elsevier Inc. All rights reserved..
Potato virus Y (PVY) is an abundant and damaging virus which reduces crop yield and marketability. Accurate detection of this economically important virus both in-field and in seed potatoes is considered essential in the control of PVY spread. Current detection methods are focused on immunodetection and PCR-based methods, however, identification of PVY through isothermal amplification is a promising avenue for developing accessible, on-site diagnostics with quick turnaround times. In this work, a rapid recombinase polymerase amplification assay was developed which could readily amplify PVY nucleic acids with good sensitivity and specificity. Additionally, this assay was shown to be capable of amplification directly from RNA in a one-step amplification process, without the need for prior reverse transcription. The assay was coupled with lateral flow technology to provide a rapid visual confirmation of amplification. This nucleic-acid lateral flow immunoassay could feasibly be employed in-field, or at any location where testing is required, to aid in the detection and control of PVY.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2022 |
|---|---|
| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:642 |
|---|---|
| Enthalten in: |
Analytical biochemistry - 642(2022) vom: 01. Apr., Seite 114526 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Cassedy, Arabelle [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Journal Article |
|---|
| Anmerkungen: |
Date Completed 09.03.2022 Date Revised 09.03.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1016/j.ab.2021.114526 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM334589851 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM334589851 | ||
| 003 | DE-627 | ||
| 005 | 20231225224031.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2022 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.ab.2021.114526 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1115.xml |
| 035 | |a (DE-627)NLM334589851 | ||
| 035 | |a (NLM)34922917 | ||
| 035 | |a (PII)S0003-2697(21)00427-9 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Cassedy, Arabelle |e verfasserin |4 aut | |
| 245 | 1 | 2 | |a A one-step reverse transcription recombinase polymerase amplification assay for lateral flow-based visual detection of PVY |
| 264 | 1 | |c 2022 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 09.03.2022 | ||
| 500 | |a Date Revised 09.03.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Copyright © 2021 Elsevier Inc. All rights reserved. | ||
| 520 | |a Potato virus Y (PVY) is an abundant and damaging virus which reduces crop yield and marketability. Accurate detection of this economically important virus both in-field and in seed potatoes is considered essential in the control of PVY spread. Current detection methods are focused on immunodetection and PCR-based methods, however, identification of PVY through isothermal amplification is a promising avenue for developing accessible, on-site diagnostics with quick turnaround times. In this work, a rapid recombinase polymerase amplification assay was developed which could readily amplify PVY nucleic acids with good sensitivity and specificity. Additionally, this assay was shown to be capable of amplification directly from RNA in a one-step amplification process, without the need for prior reverse transcription. The assay was coupled with lateral flow technology to provide a rapid visual confirmation of amplification. This nucleic-acid lateral flow immunoassay could feasibly be employed in-field, or at any location where testing is required, to aid in the detection and control of PVY | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Lateral flow | |
| 650 | 4 | |a Potato virus Y | |
| 650 | 4 | |a Rapid diagnostics | |
| 650 | 4 | |a Recombinase polymerase amplification | |
| 650 | 4 | |a Reverse transcription | |
| 650 | 7 | |a RNA, Viral |2 NLM | |
| 700 | 1 | |a Della Bartola, Michele |e verfasserin |4 aut | |
| 700 | 1 | |a Parle-McDermott, Anne |e verfasserin |4 aut | |
| 700 | 1 | |a Mullins, Ewen |e verfasserin |4 aut | |
| 700 | 1 | |a O'Kennedy, Richard |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Analytical biochemistry |d 1960 |g 642(2022) vom: 01. Apr., Seite 114526 |w (DE-627)NLM000012556 |x 1096-0309 |7 nnns |
| 773 | 1 | 8 | |g volume:642 |g year:2022 |g day:01 |g month:04 |g pages:114526 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1016/j.ab.2021.114526 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 642 |j 2022 |b 01 |c 04 |h 114526 | ||