Looking for a needle in a haystack. SARS-CoV-2 variant characterization in sewage
© 2021 The Authors..
SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:24 |
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| Enthalten in: |
Current opinion in environmental science & health - 24(2021) vom: 24. Dez., Seite 100308 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Itarte, Marta [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Revised 02.04.2022 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1016/j.coesh.2021.100308 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective | ||
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| 700 | 1 | |a Martínez-Puchol, Sandra |e verfasserin |4 aut | |
| 700 | 1 | |a Torrell, Helena |e verfasserin |4 aut | |
| 700 | 1 | |a Ceretó, Adrià |e verfasserin |4 aut | |
| 700 | 1 | |a Carrasco, Marina |e verfasserin |4 aut | |
| 700 | 1 | |a Forés, Eva |e verfasserin |4 aut | |
| 700 | 1 | |a Canela, Núria |e verfasserin |4 aut | |
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