Translational demand is not a major source of plasmid-associated fitness costs
Plasmids are key drivers of bacterial evolution because they are crucial agents for the horizontal transfer of adaptive traits, such as antibiotic resistance. Most plasmids entail a metabolic burden that reduces the fitness of their host if there is no selection for plasmid-encoded genes. It has been hypothesized that the translational demand imposed by plasmid-encoded genes is a major mechanism driving the fitness cost of plasmids. Plasmid-encoded genes typically present a different codon usage from host chromosomal genes. As a consequence, the translation of plasmid-encoded genes might sequestrate ribosomes on plasmid transcripts, overwhelming the translation machinery of the cell. However, the pervasiveness and origins of the translation-derived costs of plasmids are yet to be assessed. Here, we systematically altered translation efficiency in the host cell to disentangle the fitness effects produced by six natural antibiotic resistance plasmids. We show that limiting translation efficiency either by reducing the number of available ribosomes or their processivity does not increase plasmid costs. Overall, our results suggest that ribosomal paucity is not a major contributor to plasmid fitness costs. This article is part of the theme issue 'The secret lives of microbial mobile genetic elements'.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:377 |
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| Enthalten in: |
Philosophical transactions of the Royal Society of London. Series B, Biological sciences - 377(2022), 1842 vom: 17. Jan., Seite 20200463 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Rodríguez-Beltrán, Jerónimo [VerfasserIn] |
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| Links: |
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| Themen: |
Codon usage |
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| Anmerkungen: |
Date Completed 31.03.2022 Date Revised 04.04.2024 published: Print-Electronic figshare: 10.6084/m9.figshare.c.5674335 Citation Status MEDLINE |
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| doi: |
10.1098/rstb.2020.0463 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
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| 245 | 1 | 0 | |a Translational demand is not a major source of plasmid-associated fitness costs |
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| 500 | |a figshare: 10.6084/m9.figshare.c.5674335 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Plasmids are key drivers of bacterial evolution because they are crucial agents for the horizontal transfer of adaptive traits, such as antibiotic resistance. Most plasmids entail a metabolic burden that reduces the fitness of their host if there is no selection for plasmid-encoded genes. It has been hypothesized that the translational demand imposed by plasmid-encoded genes is a major mechanism driving the fitness cost of plasmids. Plasmid-encoded genes typically present a different codon usage from host chromosomal genes. As a consequence, the translation of plasmid-encoded genes might sequestrate ribosomes on plasmid transcripts, overwhelming the translation machinery of the cell. However, the pervasiveness and origins of the translation-derived costs of plasmids are yet to be assessed. Here, we systematically altered translation efficiency in the host cell to disentangle the fitness effects produced by six natural antibiotic resistance plasmids. We show that limiting translation efficiency either by reducing the number of available ribosomes or their processivity does not increase plasmid costs. Overall, our results suggest that ribosomal paucity is not a major contributor to plasmid fitness costs. This article is part of the theme issue 'The secret lives of microbial mobile genetic elements' | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a codon usage | |
| 650 | 4 | |a fitness cost | |
| 650 | 4 | |a horizontal gene transfer | |
| 650 | 4 | |a plasmid | |
| 650 | 4 | |a ribosome | |
| 650 | 4 | |a translational demand | |
| 700 | 1 | |a León-Sampedro, Ricardo |e verfasserin |4 aut | |
| 700 | 1 | |a Ramiro-Martínez, Paula |e verfasserin |4 aut | |
| 700 | 1 | |a de la Vega, Carmen |e verfasserin |4 aut | |
| 700 | 1 | |a Baquero, Fernando |e verfasserin |4 aut | |
| 700 | 1 | |a Levin, Bruce R |e verfasserin |4 aut | |
| 700 | 1 | |a San Millán, Álvaro |e verfasserin |4 aut | |
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