miR-494 promotes islet β cell proliferation, inhibits apoptosis and increases insulin secretion by activating Wnt/β-catenin signaling pathway
Objective To investigate the relation between the miR-494 expression with pancreatic islets β cell function and gestational diabetes mellitus. Methods Twenty patients with gestational diabetes mellitus and healthy subjects were enrolled. The content of miR-494 in peripheral blood was measured by reverse transcription PCR. INS-1 cells were cultured and treated with low glucose (3.3 mmol/L) and high glucose (16.7 mmol/L), respectively. The insulin concentration was tested by ELISA to evaluate the insulin secretion of islet cells stimulated by high glucose. Cells were collected, after treated with miR-494 mimics control, miR-494 mimics, miR-494 inhibitor control and miR-494 inhibitor for 24 hours, 48 hours and 72 hours, respectively. The activity of INS-1 cells was detected by MTT assay; Apoptosis was detected by flow cytometry. Reverse transcription PCR and Western blot analysis were used to detect the mRNA and protein expression of Wnt3a, β-catenin, cyclin D1 and c-Myc, respectively. Results Compared with the normal control, fasting insulin, fasting blood glucose, 1 hour-blood glucose and 2 hour-blood glucose in patients with gestational diabetes mellitus increased significantly. The content of miR-494 in peripheral blood decreased. The insulin concentration in the supernatant of INS-1 cells overexpressing miR-494 increased. When high glucose was given, the overexpression of miR-494 further promoted insulin secretion. Overexpression of miR-494 significantly promoted INS-1 cell activity and inhibited INS-1 cell apoptosis. miR-494 significantly promoted the protein expression of Wnt3a, β-catenin, cyclin D1 and c-Myc. miR-494 inhibitor treatment showed the opposite results. Conclusion miR-494 promotes islet β cell proliferation, inhibits apoptosis and increases insulin secretion by activating Wnt/β-catenin signaling pathway.
| Medienart: |
Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:37 |
|---|---|
| Enthalten in: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology - 37(2021), 11 vom: 23. Nov., Seite 1003-1009 |
| Sprache: |
Chinesisch |
|---|
| Beteiligte Personen: |
Song, Shanshan [VerfasserIn] |
|---|
| Themen: |
|---|
| Anmerkungen: |
Date Completed 24.11.2021 Date Revised 31.05.2022 published: Print Citation Status MEDLINE |
|---|
| Förderinstitution / Projekttitel: |
|
|---|
| PPN (Katalog-ID): |
NLM333470524 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM333470524 | ||
| 003 | DE-627 | ||
| 005 | 20231225221723.0 | ||
| 007 | tu | ||
| 008 | 231225s2021 xx ||||| 00| ||chi c | ||
| 028 | 5 | 2 | |a pubmed24n1111.xml |
| 035 | |a (DE-627)NLM333470524 | ||
| 035 | |a (NLM)34809740 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a chi | ||
| 100 | 1 | |a Song, Shanshan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a miR-494 promotes islet β cell proliferation, inhibits apoptosis and increases insulin secretion by activating Wnt/β-catenin signaling pathway |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a Date Completed 24.11.2021 | ||
| 500 | |a Date Revised 31.05.2022 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a Objective To investigate the relation between the miR-494 expression with pancreatic islets β cell function and gestational diabetes mellitus. Methods Twenty patients with gestational diabetes mellitus and healthy subjects were enrolled. The content of miR-494 in peripheral blood was measured by reverse transcription PCR. INS-1 cells were cultured and treated with low glucose (3.3 mmol/L) and high glucose (16.7 mmol/L), respectively. The insulin concentration was tested by ELISA to evaluate the insulin secretion of islet cells stimulated by high glucose. Cells were collected, after treated with miR-494 mimics control, miR-494 mimics, miR-494 inhibitor control and miR-494 inhibitor for 24 hours, 48 hours and 72 hours, respectively. The activity of INS-1 cells was detected by MTT assay; Apoptosis was detected by flow cytometry. Reverse transcription PCR and Western blot analysis were used to detect the mRNA and protein expression of Wnt3a, β-catenin, cyclin D1 and c-Myc, respectively. Results Compared with the normal control, fasting insulin, fasting blood glucose, 1 hour-blood glucose and 2 hour-blood glucose in patients with gestational diabetes mellitus increased significantly. The content of miR-494 in peripheral blood decreased. The insulin concentration in the supernatant of INS-1 cells overexpressing miR-494 increased. When high glucose was given, the overexpression of miR-494 further promoted insulin secretion. Overexpression of miR-494 significantly promoted INS-1 cell activity and inhibited INS-1 cell apoptosis. miR-494 significantly promoted the protein expression of Wnt3a, β-catenin, cyclin D1 and c-Myc. miR-494 inhibitor treatment showed the opposite results. Conclusion miR-494 promotes islet β cell proliferation, inhibits apoptosis and increases insulin secretion by activating Wnt/β-catenin signaling pathway | ||
| 650 | 4 | |a Journal Article | |
| 650 | 7 | |a MIRN494 microRNA, human |2 NLM | |
| 650 | 7 | |a MicroRNAs |2 NLM | |
| 700 | 1 | |a Yang, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a He, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Tang, Junfeng |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology |d 2003 |g 37(2021), 11 vom: 23. Nov., Seite 1003-1009 |w (DE-627)NLM148235522 |x 1007-8738 |7 nnns |
| 773 | 1 | 8 | |g volume:37 |g year:2021 |g number:11 |g day:23 |g month:11 |g pages:1003-1009 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 37 |j 2021 |e 11 |b 23 |c 11 |h 1003-1009 | ||