Regulatory function of the novel transcription factor CxrC in Penicillium oxalicum
© 2021 John Wiley & Sons Ltd..
Numerous transcription factors (TFs) in ascomycete fungi play crucial roles in cellular processes; however, how most of them function is poorly understood. Here, we identified and characterized a novel TF, CxrC (POX01387), acting downstream of the key TF CxrA, which is essential for plant-biomass-degrading-enzyme (PBDE) production in Penicillium oxalicum. Deletion of cxrC in P. oxalicum significantly affected the production of PBDEs, as well as mycelial growth and conidiospore production. CxrA directly repressed the expression of cxrC after about 12 hr following switch to Avicel culture. CxrC bound the promoters of major PBDE genes and genes involved in conidiospore development. CxrC was found to bind the TSSGTYR core sequence (S: C and G; Y: T and C; R: G and A) of the important cellulase genes cbh1 and eg1. Both N- and C-terminal peptides of CxrC and the CxrC phosphorylation were found to mediate its homodimerization. The conserved motif LPSVRSLLTP (65-74) in CxrC was found to be required for regulating cellulase production. This study reveals novel mechanisms of TF-mediated regulation of the expression of PBDE genes and genes involved in cellular processes in an ascomycete fungus.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:116 |
|---|---|
| Enthalten in: |
Molecular microbiology - 116(2021), 6 vom: 15. Dez., Seite 1512-1532 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Zhang, Ting [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Cellulase |
|---|
| Anmerkungen: |
Date Completed 25.02.2022 Date Revised 25.02.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1111/mmi.14843 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM333343859 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM333343859 | ||
| 003 | DE-627 | ||
| 005 | 20231225221455.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1111/mmi.14843 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1111.xml |
| 035 | |a (DE-627)NLM333343859 | ||
| 035 | |a (NLM)34797006 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Zhang, Ting |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Regulatory function of the novel transcription factor CxrC in Penicillium oxalicum |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 25.02.2022 | ||
| 500 | |a Date Revised 25.02.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 John Wiley & Sons Ltd. | ||
| 520 | |a Numerous transcription factors (TFs) in ascomycete fungi play crucial roles in cellular processes; however, how most of them function is poorly understood. Here, we identified and characterized a novel TF, CxrC (POX01387), acting downstream of the key TF CxrA, which is essential for plant-biomass-degrading-enzyme (PBDE) production in Penicillium oxalicum. Deletion of cxrC in P. oxalicum significantly affected the production of PBDEs, as well as mycelial growth and conidiospore production. CxrA directly repressed the expression of cxrC after about 12 hr following switch to Avicel culture. CxrC bound the promoters of major PBDE genes and genes involved in conidiospore development. CxrC was found to bind the TSSGTYR core sequence (S: C and G; Y: T and C; R: G and A) of the important cellulase genes cbh1 and eg1. Both N- and C-terminal peptides of CxrC and the CxrC phosphorylation were found to mediate its homodimerization. The conserved motif LPSVRSLLTP (65-74) in CxrC was found to be required for regulating cellulase production. This study reveals novel mechanisms of TF-mediated regulation of the expression of PBDE genes and genes involved in cellular processes in an ascomycete fungus | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a Penicillium oxalicum | |
| 650 | 4 | |a plant-biomass-degrading enzymes | |
| 650 | 4 | |a transcription factor CxrC | |
| 650 | 7 | |a Fungal Proteins |2 NLM | |
| 650 | 7 | |a Transcription Factors |2 NLM | |
| 650 | 7 | |a Cellulase |2 NLM | |
| 650 | 7 | |a EC 3.2.1.4 |2 NLM | |
| 700 | 1 | |a Mai, Rong-Ming |e verfasserin |4 aut | |
| 700 | 1 | |a Fang, Qi-Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Ou, Jian-Feng |e verfasserin |4 aut | |
| 700 | 1 | |a Mo, Li-Xiang |e verfasserin |4 aut | |
| 700 | 1 | |a Tian, Di |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Cheng-Xi |e verfasserin |4 aut | |
| 700 | 1 | |a Gu, Li-Sha |e verfasserin |4 aut | |
| 700 | 1 | |a Luo, Xue-Mei |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Jia-Xun |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Shuai |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Molecular microbiology |d 1987 |g 116(2021), 6 vom: 15. Dez., Seite 1512-1532 |w (DE-627)NLM012594180 |x 1365-2958 |7 nnns |
| 773 | 1 | 8 | |g volume:116 |g year:2021 |g number:6 |g day:15 |g month:12 |g pages:1512-1532 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1111/mmi.14843 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 116 |j 2021 |e 6 |b 15 |c 12 |h 1512-1532 | ||