Biosynthesis of l-phosphinothricin with enzymes from chromosomal integrated expression in E. coli
© King Abdulaziz City for Science and Technology 2021..
Phosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of which only the l-phosphinothricin (L-PPT) gives herbicidal function. Synthesis of optically pure L-PPT by deracemization of D/L-PPT is a promising way to cut down the environmental burden and manufacturing cost. To convert D/L-PPT to L-PPT, we expressed the catalytic enzymes by genomic integration in E. coli. The whole production was implemented in two steps in one pot using four catalytic enzymes, namely d-amino acid oxidase, catalase, glutamate dehydrogenase, and glucose dehydrogenase. Finally, after a series of process optimization, the results showed that with our system the overall L-PPT yield reached 86%. Our study demonstrated a new strategy for L-PPT synthesis, based on enzymes from chromosomal integrated expression, which does not depend on antibiotic selection, and shows a high potential for future industrial application.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
3 Biotech - 11(2021), 11 vom: 27. Nov., Seite 477 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Cen, Yu-Ke [VerfasserIn] |
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| Links: |
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| Themen: |
Biocatalysis |
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| Anmerkungen: |
Date Revised 02.11.2022 published: Print-Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1007/s13205-021-03037-7 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM333156889 |
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| 520 | |a © King Abdulaziz City for Science and Technology 2021. | ||
| 520 | |a Phosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of which only the l-phosphinothricin (L-PPT) gives herbicidal function. Synthesis of optically pure L-PPT by deracemization of D/L-PPT is a promising way to cut down the environmental burden and manufacturing cost. To convert D/L-PPT to L-PPT, we expressed the catalytic enzymes by genomic integration in E. coli. The whole production was implemented in two steps in one pot using four catalytic enzymes, namely d-amino acid oxidase, catalase, glutamate dehydrogenase, and glucose dehydrogenase. Finally, after a series of process optimization, the results showed that with our system the overall L-PPT yield reached 86%. Our study demonstrated a new strategy for L-PPT synthesis, based on enzymes from chromosomal integrated expression, which does not depend on antibiotic selection, and shows a high potential for future industrial application | ||
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| 650 | 4 | |a Deracemization | |
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| 700 | 1 | |a Xue, Ya-Ping |e verfasserin |4 aut | |
| 700 | 1 | |a Zheng, Yu-Guo |e verfasserin |4 aut | |
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