Preventing c2c12 muscular cells damage combining magnesium and potassium with vitamin D3 and curcumin

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BACKGROUND AND AIM: Physical activity is defined as any bodily movement produced by skeletal muscles which causes energy consumption; moderate and constant physical activity is known to be beneficial and to slow the muscle loss process associated with aging. The aim of the present study was to test, in an in vitro exercise model, the biological effects of a new formulation composed of magnesium and potassium combined with vitamin D and curcumin created to support muscle activity and to prevent hypercontraction damage.

EXPERIMENTAL PROCEDURE: C2C12 cells were treated with vitamin D, buffered magnesium bisglycinate, curcumin, and potassium citrate. Cell viability, morpho-functional changes, calcium and magnesium movements, and the main kinases involved in glucose uptake were analyzed. The glycogen level and lactate were also evaluated.

RESULTS AND CONCLUSION: Important results about a positive effect on mitochondrial activity, ATP production, oxygen consumption and in the physiological differentiation of C2C12 cells were obtained. Further experiments were performed under conditions that mimic the biological aspects of strenuous exercise. The combination of magnesium, vitamin D3, curcumin, and potassium citrate revealed beneficial effects on skeletal muscle cells under physiological conditions as well as while mimicking intense activity. In particular, in an in vitro model, they were able to control the hypercontraction, restoring ion fluxes, reducing inflammation signaling and supporting the main mechanism involved on aerobic activity. Our results have indicated for the first time that this new combination could be considered as a new nutraceutical formulation to improve physical performance and muscle recovery.

Medienart:

E-Artikel

Erscheinungsjahr:

2021

Erschienen:

2021

Enthalten in:

Zur Gesamtaufnahme - volume:11

Enthalten in:

Journal of traditional and complementary medicine - 11(2021), 6 vom: 01. Nov., Seite 532-544

Sprache:

Englisch

Beteiligte Personen:

Molinari, Claudio [VerfasserIn]
Ruga, Sara [VerfasserIn]
Farghali, Mahitab [VerfasserIn]
Galla, Rebecca [VerfasserIn]
Bassiouny, Ahmad [VerfasserIn]
Uberti, Francesca [VerfasserIn]

Links:

Volltext

Themen:

C2C12 cells
Fatigue
Journal Article
Muscle recovery
Nutraceuticals
Physical activity

Anmerkungen:

Date Revised 28.04.2022

published: Electronic-eCollection

Citation Status PubMed-not-MEDLINE

doi:

10.1016/j.jtcme.2021.05.003

funding:

Förderinstitution / Projekttitel:

PPN (Katalog-ID):

NLM333034139