Novel 3D capsule device to restrict kidney volume expansion on polycystic kidney progression : feasibility study in a rat model
© 2021. Italian Society of Nephrology..
BACKGROUND: Cystogenesis in polycystic kidney disease (PKD) is likely accelerated by various renal insults, including crystal deposition, that activate renal tubule obstruction and dilation. We developed a capsule-based device that can be applied to cystic kidneys to restrict tubular lumen dilatation and cyst expansion.
METHODS: Kidney capsule devices were designed from computed tomography images of wild-type and Cy/+ rats. Capsule devices were surgically implanted on kidneys in six surgical sessions over a period of 14 months in 7 wild-type rats of 6.5-8 weeks (3 sham operations, 2 right, 2 left) and 6 Cy/+ rats of 6.5 weeks (2 sham, 3 left, 1 bilateral). After surgery, the rats were followed for 5.4-12.4 weeks' growth and sacrificed to retrieve the kidneys. During the follow-up, serum creatinine was measured and retrieved kidneys were weighed. Histological analysis including cystic area measurement and immunohistochemistry was performed.
RESULTS: Morphometric capsule devices were configured and developed by an image processing technique and produced using a 3D printer. Encapsulated Cy/+ kidneys (n = 5; mean weight 3.64 g) were consistently smaller in size (by 21-36%; p < 0.001) than unencapsulated Cy/+ kidneys (n = 7; mean weight 5.52 g). Encapsulated Cy/+ kidneys (mean %cyst area: 29.4%) showed smaller histological cystic area (by 28-58%; p < 0.001) than unencapsulated Cy/+ kidneys (mean %cyst area 48.6%). Cell proliferation and macrophages were also markedly reduced in encapsulated Cy/+ kidneys, compared to unencapsulated Cy/+ kidneys.
CONCLUSIONS: We report a pilot feasibility study for the application of a novel morphometric 3D capsule device to the Cy/+ rat model showing restricted kidney volume expansion on polycystic kidney disease progression.
| Errataetall: |
CommentIn: J Nephrol. 2023 Apr;36(3):933-934. - PMID 35960431 |
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| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:35 |
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| Enthalten in: |
Journal of nephrology - 35(2022), 3 vom: 10. Apr., Seite 1033-1040 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Bae, Kyongtae T [VerfasserIn] |
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| Links: |
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| Themen: |
3D printing |
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| Anmerkungen: |
Date Completed 12.04.2022 Date Revised 20.04.2023 published: Print-Electronic CommentIn: J Nephrol. 2023 Apr;36(3):933-934. - PMID 35960431 Citation Status MEDLINE |
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| doi: |
10.1007/s40620-021-01160-5 |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM332955117 |
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| 245 | 1 | 0 | |a Novel 3D capsule device to restrict kidney volume expansion on polycystic kidney progression |b feasibility study in a rat model |
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| 500 | |a Date Revised 20.04.2023 | ||
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| 500 | |a CommentIn: J Nephrol. 2023 Apr;36(3):933-934. - PMID 35960431 | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021. Italian Society of Nephrology. | ||
| 520 | |a BACKGROUND: Cystogenesis in polycystic kidney disease (PKD) is likely accelerated by various renal insults, including crystal deposition, that activate renal tubule obstruction and dilation. We developed a capsule-based device that can be applied to cystic kidneys to restrict tubular lumen dilatation and cyst expansion | ||
| 520 | |a METHODS: Kidney capsule devices were designed from computed tomography images of wild-type and Cy/+ rats. Capsule devices were surgically implanted on kidneys in six surgical sessions over a period of 14 months in 7 wild-type rats of 6.5-8 weeks (3 sham operations, 2 right, 2 left) and 6 Cy/+ rats of 6.5 weeks (2 sham, 3 left, 1 bilateral). After surgery, the rats were followed for 5.4-12.4 weeks' growth and sacrificed to retrieve the kidneys. During the follow-up, serum creatinine was measured and retrieved kidneys were weighed. Histological analysis including cystic area measurement and immunohistochemistry was performed | ||
| 520 | |a RESULTS: Morphometric capsule devices were configured and developed by an image processing technique and produced using a 3D printer. Encapsulated Cy/+ kidneys (n = 5; mean weight 3.64 g) were consistently smaller in size (by 21-36%; p < 0.001) than unencapsulated Cy/+ kidneys (n = 7; mean weight 5.52 g). Encapsulated Cy/+ kidneys (mean %cyst area: 29.4%) showed smaller histological cystic area (by 28-58%; p < 0.001) than unencapsulated Cy/+ kidneys (mean %cyst area 48.6%). Cell proliferation and macrophages were also markedly reduced in encapsulated Cy/+ kidneys, compared to unencapsulated Cy/+ kidneys | ||
| 520 | |a CONCLUSIONS: We report a pilot feasibility study for the application of a novel morphometric 3D capsule device to the Cy/+ rat model showing restricted kidney volume expansion on polycystic kidney disease progression | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a 3D printing | |
| 650 | 4 | |a Image-processing | |
| 650 | 4 | |a Intervention | |
| 650 | 4 | |a Polycystic kidney disease | |
| 650 | 4 | |a Rodent experiment | |
| 700 | 1 | |a Kumamoto, Kanako |e verfasserin |4 aut | |
| 700 | 1 | |a Yoshimura, Aya |e verfasserin |4 aut | |
| 700 | 1 | |a Kugita, Masanori |e verfasserin |4 aut | |
| 700 | 1 | |a Horie, Shigeo |e verfasserin |4 aut | |
| 700 | 1 | |a Yamaguchi, Tamio |e verfasserin |4 aut | |
| 700 | 1 | |a Bae, Junu T |e verfasserin |4 aut | |
| 700 | 1 | |a Nagao, Shizuko |e verfasserin |4 aut | |
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