Fiber Formation and Mechanical Properties of Bombyx mori Silk Are Regulated by Vacuolar-Type ATPase
The mechanism of silk fiber formation in silkworms, Bombyx mori, is of particular scientific interest because it is closely related to the mechanical properties of silk fibers. However, there are still substantial knowledge gaps in understanding the details of this mechanism. Studies have found a pH gradient in the silk gland of silkworms. A vacuolar-type ATPase (V-ATPase) is thought to be involved in establishing this pH gradient. Although it is reported that the pH gradient plays a role in silk fibrillogenesis, the direct relationship between V-ATPase and silk mechanical properties is unclear. Thus, this study aims to clarify this relationship. We found that V-ATPase is highly and stably expressed in the anterior silk gland (ASG) and maintains the pH gradient and the fine structure of ASG. Inhibition of V-ATPase activity increased the β-sheet content and crystallinity of silk fibers. Tensile testing showed that the mechanical properties of silk fibers improved after inhibiting V-ATPase activity. All the data suggest that V-ATPase is a key factor in regulating silk fibrillogenesis and is related to the final mechanical properties of the silk fibers. V-ATPase is a potential target for silk mechanical property improvement.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
|---|---|
| Enthalten in: |
ACS biomaterials science & engineering - 7(2021), 12 vom: 13. Dez., Seite 5532-5540 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Wang, Xin [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Bafilomycin A1 |
|---|
| Anmerkungen: |
Date Completed 25.01.2022 Date Revised 25.01.2022 published: Print-Electronic Citation Status MEDLINE |
|---|
| doi: |
10.1021/acsbiomaterials.1c01230 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM33291268X |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM33291268X | ||
| 003 | DE-627 | ||
| 005 | 20231225220543.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1021/acsbiomaterials.1c01230 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1109.xml |
| 035 | |a (DE-627)NLM33291268X | ||
| 035 | |a (NLM)34753284 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Wang, Xin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Fiber Formation and Mechanical Properties of Bombyx mori Silk Are Regulated by Vacuolar-Type ATPase |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Completed 25.01.2022 | ||
| 500 | |a Date Revised 25.01.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a The mechanism of silk fiber formation in silkworms, Bombyx mori, is of particular scientific interest because it is closely related to the mechanical properties of silk fibers. However, there are still substantial knowledge gaps in understanding the details of this mechanism. Studies have found a pH gradient in the silk gland of silkworms. A vacuolar-type ATPase (V-ATPase) is thought to be involved in establishing this pH gradient. Although it is reported that the pH gradient plays a role in silk fibrillogenesis, the direct relationship between V-ATPase and silk mechanical properties is unclear. Thus, this study aims to clarify this relationship. We found that V-ATPase is highly and stably expressed in the anterior silk gland (ASG) and maintains the pH gradient and the fine structure of ASG. Inhibition of V-ATPase activity increased the β-sheet content and crystallinity of silk fibers. Tensile testing showed that the mechanical properties of silk fibers improved after inhibiting V-ATPase activity. All the data suggest that V-ATPase is a key factor in regulating silk fibrillogenesis and is related to the final mechanical properties of the silk fibers. V-ATPase is a potential target for silk mechanical property improvement | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 4 | |a bafilomycin A1 | |
| 650 | 4 | |a mechanical properties | |
| 650 | 4 | |a silk fiber | |
| 650 | 4 | |a silk fibrillogenesis | |
| 650 | 4 | |a vacuolar-type ATPase | |
| 650 | 7 | |a Silk |2 NLM | |
| 650 | 7 | |a Vacuolar Proton-Translocating ATPases |2 NLM | |
| 650 | 7 | |a EC 3.6.1.- |2 NLM | |
| 700 | 1 | |a Tan, Xiaoyin |e verfasserin |4 aut | |
| 700 | 1 | |a Liu, Qingsong |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Yi |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xinning |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Zhaoming |e verfasserin |4 aut | |
| 700 | 1 | |a Dong, Haonan |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Qingyou |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Ping |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t ACS biomaterials science & engineering |d 2015 |g 7(2021), 12 vom: 13. Dez., Seite 5532-5540 |w (DE-627)NLM249114623 |x 2373-9878 |7 nnns |
| 773 | 1 | 8 | |g volume:7 |g year:2021 |g number:12 |g day:13 |g month:12 |g pages:5532-5540 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.1021/acsbiomaterials.1c01230 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 7 |j 2021 |e 12 |b 13 |c 12 |h 5532-5540 | ||