Effects of fine particulate matter on cognitive function and gut microbiota in adult male mice
OBJECTIVE: To study the effects of fine particulate matter(PM_(2.5))exposure to cognitive function and intestinal flora abundance and diversity in adult male mice.
METHODS: The SPF grade male C57 BL/6 J mice with 8 weeks old were randomly divided into control group(NS group), PM_(2.5) exposure group(PM_(2.5) group), probiotic group(VSL#3 group) and PM_(2.5) + VSL#3 group(PMV group), with 8 mice in each group. The PM_(2.5) group and PMV group mice were exposed to PM_(2.5) using animal exposure system equipped with real-time PM_(2.5) concentration, and concentrated 6 times the outdoor PM_(2.5) concentration, 8 h every day, 5 d every week for 4 weeks. The VSL#3 group and PMV group mice were given VSL#3, 0.5 mL, 2×10~9 CFU/mL. After four weeks of exposure, feces from mice were collected for 16 s rRNA high-throughput sequencing, and the cognitive function was evaluated using Morris water maze and object recognition experiments.
RESULTS: The escape latency of PM_(2.5) group in four-day training [(54.99±6.77) s, (41.21±9.98) s, (36.27±13.11) s, (30.01±14.80) s] were higher than that of NS group [(32.19±4.59) s, (20.50±6.77) s, (19.93±7.30) s, (16.94±9.91) s], and the difference were statistically significant(P<0.05). The escape latency of PMV group on the first and second day of training [(39.02±6.23) s, (28.83±9.53) s] were lower than that of PM_(2.5) group(P<0.05). The target quadrant residence time of mice in PM_(2.5) group [(18.30± 8.88) s] was lower than that in NS group and PMV group [(30.53±9.10) s, (30.00±10.61) s]. Compared with NS group(6.09±0.40), the shannon index of PM_(2.5) group and PMV group(5.05±0.65 and 5.46±0.52) were significantly reduced(P<0.05). The target quadrant time was positively correlated with the relative abundance of Actinomyces(r=0.576, P<0.05), and the recognition index was positively correlated with the relative abundance of Firmicutes(r=0.612, P<0.05).
CONCLUSION: PM_(2.5) could lead to cognitive dysfunction in mice, which is related to diversity and abundance of the intestinal flora. Probiotic can improve cognitive function.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:50 |
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| Enthalten in: |
Wei sheng yan jiu = Journal of hygiene research - 50(2021), 5 vom: 01. Sept., Seite 821-826 |
| Sprache: |
Chinesisch |
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| Beteiligte Personen: |
Wu, Yanfang [VerfasserIn] |
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| Links: |
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| Themen: |
Cognitive function |
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| Anmerkungen: |
Date Completed 10.11.2021 Date Revised 10.11.2021 published: Print Citation Status MEDLINE |
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| doi: |
10.19813/j.cnki.weishengyanjiu.2021.05.019 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM332879054 |
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| 245 | 1 | 0 | |a Effects of fine particulate matter on cognitive function and gut microbiota in adult male mice |
| 264 | 1 | |c 2021 | |
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| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
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| 500 | |a Date Completed 10.11.2021 | ||
| 500 | |a Date Revised 10.11.2021 | ||
| 500 | |a published: Print | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a OBJECTIVE: To study the effects of fine particulate matter(PM_(2.5))exposure to cognitive function and intestinal flora abundance and diversity in adult male mice | ||
| 520 | |a METHODS: The SPF grade male C57 BL/6 J mice with 8 weeks old were randomly divided into control group(NS group), PM_(2.5) exposure group(PM_(2.5) group), probiotic group(VSL#3 group) and PM_(2.5) + VSL#3 group(PMV group), with 8 mice in each group. The PM_(2.5) group and PMV group mice were exposed to PM_(2.5) using animal exposure system equipped with real-time PM_(2.5) concentration, and concentrated 6 times the outdoor PM_(2.5) concentration, 8 h every day, 5 d every week for 4 weeks. The VSL#3 group and PMV group mice were given VSL#3, 0.5 mL, 2×10~9 CFU/mL. After four weeks of exposure, feces from mice were collected for 16 s rRNA high-throughput sequencing, and the cognitive function was evaluated using Morris water maze and object recognition experiments | ||
| 520 | |a RESULTS: The escape latency of PM_(2.5) group in four-day training [(54.99±6.77) s, (41.21±9.98) s, (36.27±13.11) s, (30.01±14.80) s] were higher than that of NS group [(32.19±4.59) s, (20.50±6.77) s, (19.93±7.30) s, (16.94±9.91) s], and the difference were statistically significant(P<0.05). The escape latency of PMV group on the first and second day of training [(39.02±6.23) s, (28.83±9.53) s] were lower than that of PM_(2.5) group(P<0.05). The target quadrant residence time of mice in PM_(2.5) group [(18.30± 8.88) s] was lower than that in NS group and PMV group [(30.53±9.10) s, (30.00±10.61) s]. Compared with NS group(6.09±0.40), the shannon index of PM_(2.5) group and PMV group(5.05±0.65 and 5.46±0.52) were significantly reduced(P<0.05). The target quadrant time was positively correlated with the relative abundance of Actinomyces(r=0.576, P<0.05), and the recognition index was positively correlated with the relative abundance of Firmicutes(r=0.612, P<0.05) | ||
| 520 | |a CONCLUSION: PM_(2.5) could lead to cognitive dysfunction in mice, which is related to diversity and abundance of the intestinal flora. Probiotic can improve cognitive function | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a cognitive function | |
| 650 | 4 | |a fine particulate matter | |
| 650 | 4 | |a intestinal flora | |
| 650 | 4 | |a probiotic | |
| 650 | 7 | |a Particulate Matter |2 NLM | |
| 700 | 1 | |a Yin, Jin |e verfasserin |4 aut | |
| 700 | 1 | |a Cao, Wa |e verfasserin |4 aut | |
| 700 | 1 | |a Kang, Huiwen |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Qian |e verfasserin |4 aut | |
| 700 | 1 | |a Bian, Jingjing |e verfasserin |4 aut | |
| 700 | 1 | |a Tong, Junwang |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Jinlong |e verfasserin |4 aut | |
| 700 | 1 | |a Jiang, Shoufang |e verfasserin |4 aut | |
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