Anatomy-oriented stereotactic approach to cerebrospinal fluid collection in mice
Copyright © 2021 Elsevier B.V. All rights reserved..
Extraction of cerebrospinal fluid (CSF) in small animal models is an indispensable technique used in neuroscience and neuro-oncology research but can be technically challenging due to the small size of the brain. Here we describe a simple, reliable, and highly reproducible method for collecting CSF from anesthetized laboratory mice based on anatomical foundation, with no requirement of introducing any custom-made types of equipment. The mouse's head is fixed to a stereotaxic frame in the position that allows the maximum opening of the cisterna magna. A Hamilton syringe attached with a 26 gauge needle is inserted stereotactically in parallel to the brain axis to reach the cistern. The clear CSF (approximately 10 µl) can then be drawn slowly in approximately 5 min. Our modified approach minimizes technical variations that can result from customized equipment, and is applicable in broad research settings.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:1774 |
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| Enthalten in: |
Brain research - 1774(2022) vom: 01. Jan., Seite 147706 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Shimizu, Kazuhide [VerfasserIn] |
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| Links: |
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| Themen: |
CSF collection |
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| Anmerkungen: |
Date Completed 28.03.2022 Date Revised 28.03.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1016/j.brainres.2021.147706 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 520 | |a Extraction of cerebrospinal fluid (CSF) in small animal models is an indispensable technique used in neuroscience and neuro-oncology research but can be technically challenging due to the small size of the brain. Here we describe a simple, reliable, and highly reproducible method for collecting CSF from anesthetized laboratory mice based on anatomical foundation, with no requirement of introducing any custom-made types of equipment. The mouse's head is fixed to a stereotaxic frame in the position that allows the maximum opening of the cisterna magna. A Hamilton syringe attached with a 26 gauge needle is inserted stereotactically in parallel to the brain axis to reach the cistern. The clear CSF (approximately 10 µl) can then be drawn slowly in approximately 5 min. Our modified approach minimizes technical variations that can result from customized equipment, and is applicable in broad research settings | ||
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| 700 | 1 | |a Wakimoto, Hiroaki |e verfasserin |4 aut | |
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