Antidiarrheal activity of Bridelia ferruginea bark methanolic extract involves modulation ATPases in mice and inhibition of muscarinic acetylcholine receptor (M3) and prostaglandin E2 receptor 3 (EP3) in silico
© 2021 Walter de Gruyter GmbH, Berlin/Boston..
OBJECTIVES: Diarrhea, an abnormal state in which the individual has about three or more daily bowel movements, is now considered one of the most challenging global public health problems. Using plant products, such as Bridelia ferruginea is an alternative treatment option. The objective of this study was to investigate the antidiarrheal activity of B. ferruginea bark methanolic extract (BfME) and the mechanisms involved.
METHODS: BfME antidiarrheal activity was evaluated in mice model of castor oil-induced diarrhea and enteropooling. To evaluate motility, gastrointestinal transit time was carried out using phenol red meal, while intestinal activities of selected ATPases were also evaluated. Furthermore, the active components in BfME were detected by GC-MS analysis, while molecular docking of the most abundant compounds with muscarinic acetylcholine receptor (M3) and prostaglandin E2 receptor 3 (EP3) were conducted.
RESULTS: BfME at 400 and 800 mg/kg showed antidiarrheal activity by delaying onset of diarrhea, reduced gastrointestinal transit and increased intestinal activities of Na+ K+-ATPase, Ca2+ Mg2+-ATPase and Mg2+-ATPase. Molecular docking revealed that γ-sitosterol, α-amyrin, and stigmasterol have outstanding binding affinity for M3 and EP3.
CONCLUSIONS: In view of these results, the observed antidiarrheal activity possibly occurs via the activation of ATPases activities and inhibition of M3 and EP3.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2023 |
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| Erschienen: |
2023 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:20 |
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| Enthalten in: |
Journal of complementary & integrative medicine - 20(2023), 4 vom: 01. Dez., Seite 757-771 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Omolaso, Blessing Olugbamila [VerfasserIn] |
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| Links: |
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| Themen: |
Bridelia ferruginea |
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| Anmerkungen: |
Date Revised 09.12.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1515/jcim-2021-0240 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM332657701 |
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| 245 | 1 | 0 | |a Antidiarrheal activity of Bridelia ferruginea bark methanolic extract involves modulation ATPases in mice and inhibition of muscarinic acetylcholine receptor (M3) and prostaglandin E2 receptor 3 (EP3) in silico |
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| 500 | |a Date Revised 09.12.2023 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a © 2021 Walter de Gruyter GmbH, Berlin/Boston. | ||
| 520 | |a OBJECTIVES: Diarrhea, an abnormal state in which the individual has about three or more daily bowel movements, is now considered one of the most challenging global public health problems. Using plant products, such as Bridelia ferruginea is an alternative treatment option. The objective of this study was to investigate the antidiarrheal activity of B. ferruginea bark methanolic extract (BfME) and the mechanisms involved | ||
| 520 | |a METHODS: BfME antidiarrheal activity was evaluated in mice model of castor oil-induced diarrhea and enteropooling. To evaluate motility, gastrointestinal transit time was carried out using phenol red meal, while intestinal activities of selected ATPases were also evaluated. Furthermore, the active components in BfME were detected by GC-MS analysis, while molecular docking of the most abundant compounds with muscarinic acetylcholine receptor (M3) and prostaglandin E2 receptor 3 (EP3) were conducted | ||
| 520 | |a RESULTS: BfME at 400 and 800 mg/kg showed antidiarrheal activity by delaying onset of diarrhea, reduced gastrointestinal transit and increased intestinal activities of Na+ K+-ATPase, Ca2+ Mg2+-ATPase and Mg2+-ATPase. Molecular docking revealed that γ-sitosterol, α-amyrin, and stigmasterol have outstanding binding affinity for M3 and EP3 | ||
| 520 | |a CONCLUSIONS: In view of these results, the observed antidiarrheal activity possibly occurs via the activation of ATPases activities and inhibition of M3 and EP3 | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Bridelia ferruginea | |
| 650 | 4 | |a castor oil | |
| 650 | 4 | |a diarrhea | |
| 650 | 4 | |a gastrointestinal transit | |
| 650 | 4 | |a mice | |
| 700 | 1 | |a Adesanwo, Julius Kolawole |e verfasserin |4 aut | |
| 700 | 1 | |a Ishola, Ahmed Adebayo |e verfasserin |4 aut | |
| 700 | 1 | |a Adegoke, Adeoti Gbemisola |e verfasserin |4 aut | |
| 700 | 1 | |a Akingbule, Francis O |e verfasserin |4 aut | |
| 700 | 1 | |a Ipadeola, Yetunde Ayoka |e verfasserin |4 aut | |
| 700 | 1 | |a Adewole, Kayode Ezekiel |e verfasserin |4 aut | |
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