Binding of the RNA Chaperone Hfq on Target mRNAs Promotes the Small RNA RyhB-Induced Degradation in Escherichia coli
Many RNA-RNA interactions depend on molecular chaperones to form and remain stable in living cells. A prime example is the RNA chaperone Hfq, which is a critical effector involved in regulatory interactions between small RNAs (sRNAs) and cognate target mRNAs in Enterobacteriaceae. While there is a great deal of in vitro biochemical evidence supporting the model that Hfq enhances rates or affinities of sRNA:mRNA interactions, there is little corroborating in vivo evidence. Here we used in vivo tools including reporter genes, co-purification assays, and super-resolution microscopy to analyze the role of Hfq in RyhB-mediated regulation, and we found that Hfq is often unnecessary for efficient RyhB:mRNA complex formation in vivo. Remarkably, our data suggest that a primary function of Hfq is to promote RyhB-induced cleavage of mRNA targets by RNase E. Moreover, our work indicates that Hfq plays a more limited role in dictating regulatory outcomes following sRNAs RybB and DsrA complex formation with specific target mRNAs. Our investigation helps evaluate the roles played by Hfq in some RNA-mediated regulation.
| Medienart: |
E-Artikel |
|---|
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:7 |
|---|---|
| Enthalten in: |
Non-coding RNA - 7(2021), 4 vom: 28. Sept. |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Lalaouna, David [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Hfq |
|---|
| Anmerkungen: |
Date Revised 17.11.2021 published: Electronic Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.3390/ncrna7040064 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM332383156 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM332383156 | ||
| 003 | DE-627 | ||
| 005 | 20231225215448.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3390/ncrna7040064 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1107.xml |
| 035 | |a (DE-627)NLM332383156 | ||
| 035 | |a (NLM)34698252 | ||
| 035 | |a (PII)64 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Lalaouna, David |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Binding of the RNA Chaperone Hfq on Target mRNAs Promotes the Small RNA RyhB-Induced Degradation in Escherichia coli |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 17.11.2021 | ||
| 500 | |a published: Electronic | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Many RNA-RNA interactions depend on molecular chaperones to form and remain stable in living cells. A prime example is the RNA chaperone Hfq, which is a critical effector involved in regulatory interactions between small RNAs (sRNAs) and cognate target mRNAs in Enterobacteriaceae. While there is a great deal of in vitro biochemical evidence supporting the model that Hfq enhances rates or affinities of sRNA:mRNA interactions, there is little corroborating in vivo evidence. Here we used in vivo tools including reporter genes, co-purification assays, and super-resolution microscopy to analyze the role of Hfq in RyhB-mediated regulation, and we found that Hfq is often unnecessary for efficient RyhB:mRNA complex formation in vivo. Remarkably, our data suggest that a primary function of Hfq is to promote RyhB-induced cleavage of mRNA targets by RNase E. Moreover, our work indicates that Hfq plays a more limited role in dictating regulatory outcomes following sRNAs RybB and DsrA complex formation with specific target mRNAs. Our investigation helps evaluate the roles played by Hfq in some RNA-mediated regulation | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Hfq | |
| 650 | 4 | |a Hfq binding site | |
| 650 | 4 | |a RNA chaperone | |
| 650 | 4 | |a RNase E | |
| 650 | 4 | |a sRNA-induced degradation | |
| 650 | 4 | |a small RNA (sRNA) | |
| 650 | 4 | |a super-resolution imaging | |
| 700 | 1 | |a Prévost, Karine |e verfasserin |4 aut | |
| 700 | 1 | |a Park, Seongjin |e verfasserin |4 aut | |
| 700 | 1 | |a Chénard, Thierry |e verfasserin |4 aut | |
| 700 | 1 | |a Bouchard, Marie-Pier |e verfasserin |4 aut | |
| 700 | 1 | |a Caron, Marie-Pier |e verfasserin |4 aut | |
| 700 | 1 | |a Vanderpool, Carin K |e verfasserin |4 aut | |
| 700 | 1 | |a Fei, Jingyi |e verfasserin |4 aut | |
| 700 | 1 | |a Massé, Eric |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Non-coding RNA |d 2014 |g 7(2021), 4 vom: 28. Sept. |w (DE-627)NLM253091705 |x 2311-553X |7 nnns |
| 773 | 1 | 8 | |g volume:7 |g year:2021 |g number:4 |g day:28 |g month:09 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3390/ncrna7040064 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 7 |j 2021 |e 4 |b 28 |c 09 | ||