An artificial chromosome for data storage
© The Author(s) 2021. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd..
DNA digital storage provides an alternative for information storage with high density and long-term stability. Here, we report the de novo design and synthesis of an artificial chromosome that encodes two pictures and a video clip. The encoding paradigm utilizing the superposition of sparsified error correction codewords and pseudo-random sequences tolerates base insertions/deletions and is well suited to error-prone nanopore sequencing for data retrieval. The entire 254 kb sequence was 95.27% occupied by encoded data. The Transformation-Associated Recombination method was used in the construction of this chromosome from DNA fragments and necessary autonomous replication sequences. The stability was demonstrated by transmitting the data-carrying chromosome to the 100th generation. This study demonstrates a data storage method using encoded artificial chromosomes via in vivo assembly for write-once and stable replication for multiple retrievals, similar to a compact disc, with potential in economically massive data distribution.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
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| Enthalten in: |
National science review - 8(2021), 5 vom: 07. Mai, Seite nwab028 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Chen, Weigang [VerfasserIn] |
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| Links: |
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| Themen: |
Artificial chromosome |
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| Anmerkungen: |
Date Revised 20.09.2023 published: Electronic-eCollection Citation Status PubMed-not-MEDLINE |
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| doi: |
10.1093/nsr/nwab028 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM332317455 |
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| 520 | |a © The Author(s) 2021. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd. | ||
| 520 | |a DNA digital storage provides an alternative for information storage with high density and long-term stability. Here, we report the de novo design and synthesis of an artificial chromosome that encodes two pictures and a video clip. The encoding paradigm utilizing the superposition of sparsified error correction codewords and pseudo-random sequences tolerates base insertions/deletions and is well suited to error-prone nanopore sequencing for data retrieval. The entire 254 kb sequence was 95.27% occupied by encoded data. The Transformation-Associated Recombination method was used in the construction of this chromosome from DNA fragments and necessary autonomous replication sequences. The stability was demonstrated by transmitting the data-carrying chromosome to the 100th generation. This study demonstrates a data storage method using encoded artificial chromosomes via in vivo assembly for write-once and stable replication for multiple retrievals, similar to a compact disc, with potential in economically massive data distribution | ||
| 650 | 4 | |a Journal Article | |
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| 650 | 4 | |a artificial chromosome | |
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| 650 | 4 | |a indel correction | |
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| 700 | 1 | |a Han, Mingzhe |e verfasserin |4 aut | |
| 700 | 1 | |a Zhou, Jianting |e verfasserin |4 aut | |
| 700 | 1 | |a Ge, Qi |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Panpan |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Xinchen |e verfasserin |4 aut | |
| 700 | 1 | |a Zhu, Siyu |e verfasserin |4 aut | |
| 700 | 1 | |a Song, Lifu |e verfasserin |4 aut | |
| 700 | 1 | |a Yuan, Yingjin |e verfasserin |4 aut | |
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