The expression pattern of CD10 and CD31 identifies fine fibrovascular stroma of grade 1-endometrial endometrioid carcinomas in cytology
© 2021 John Wiley & Sons Ltd..
INTRODUCTION: The objective of this study was to assess the diagnostic utility of CD10 in the differential diagnosis of grade 1-endometrial endometrioid carcinoma (G1-EEC) and the metaplastic changes associated with the endometrial glandular and stromal breakdown (EGBD) on liquid-based cytological (LBC) samples.
METHODS: (1) The type and distribution of CD10-positive cells in EGBD and G1-EEC patients were evaluated. (2) Based on the results from (1), histological and cytological specimens were double-immunostained with CD31 and CD10 to confirm whether CD10-positive tubular-canalicular material found in (1) was represented by fine threads of endometrial-type fibrovascular stroma. (3) Based on the results from (2), additional immunostaining of histological specimens was performed for CD146 and αSMA as markers of perivascular cells.
RESULTS: (1) CD10 positive cells showed two main patterns of expression: cytoplasmic immunoreactivity in the form of dense brown granules in EGBD and tubular-canalicular branching patterns in G1-EEC. (2) The tubular-canalicular material observed in cytological specimens of G1-EEC samples co-expressed CD10 and CD31, and was interpreted as representing fine threads of endometrial fibrovascular stroma in the corresponding histological samples. Conversely, metaplastic changes in EGBD cases, only a few CD31-positive signals were found inside the condensed stromal clusters with CD10-positive. (3) Cells surrounding the CD31-positive vascular endothelial cells expressed CD146 and αSMA; moreover, some of the thin CD10-positive fibrous stromal strands also co-expressed αSMA.
CONCLUSIONS: CD10 is a very useful immunomarker for distinguishing between G1-EEC and the metaplastic changes of EGBD in LBC samples.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2022 |
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| Erschienen: |
2022 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:33 |
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| Enthalten in: |
Cytopathology : official journal of the British Society for Clinical Cytology - 33(2022), 3 vom: 15. Mai, Seite 362-373 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Norimatsu, Yoshiaki [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 12.04.2022 Date Revised 22.06.2022 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1111/cyt.13070 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM332294773 |
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| 041 | |a eng | ||
| 100 | 1 | |a Norimatsu, Yoshiaki |e verfasserin |4 aut | |
| 245 | 1 | 4 | |a The expression pattern of CD10 and CD31 identifies fine fibrovascular stroma of grade 1-endometrial endometrioid carcinomas in cytology |
| 264 | 1 | |c 2022 | |
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| 500 | |a Date Completed 12.04.2022 | ||
| 500 | |a Date Revised 22.06.2022 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 John Wiley & Sons Ltd. | ||
| 520 | |a INTRODUCTION: The objective of this study was to assess the diagnostic utility of CD10 in the differential diagnosis of grade 1-endometrial endometrioid carcinoma (G1-EEC) and the metaplastic changes associated with the endometrial glandular and stromal breakdown (EGBD) on liquid-based cytological (LBC) samples | ||
| 520 | |a METHODS: (1) The type and distribution of CD10-positive cells in EGBD and G1-EEC patients were evaluated. (2) Based on the results from (1), histological and cytological specimens were double-immunostained with CD31 and CD10 to confirm whether CD10-positive tubular-canalicular material found in (1) was represented by fine threads of endometrial-type fibrovascular stroma. (3) Based on the results from (2), additional immunostaining of histological specimens was performed for CD146 and αSMA as markers of perivascular cells | ||
| 520 | |a RESULTS: (1) CD10 positive cells showed two main patterns of expression: cytoplasmic immunoreactivity in the form of dense brown granules in EGBD and tubular-canalicular branching patterns in G1-EEC. (2) The tubular-canalicular material observed in cytological specimens of G1-EEC samples co-expressed CD10 and CD31, and was interpreted as representing fine threads of endometrial fibrovascular stroma in the corresponding histological samples. Conversely, metaplastic changes in EGBD cases, only a few CD31-positive signals were found inside the condensed stromal clusters with CD10-positive. (3) Cells surrounding the CD31-positive vascular endothelial cells expressed CD146 and αSMA; moreover, some of the thin CD10-positive fibrous stromal strands also co-expressed αSMA | ||
| 520 | |a CONCLUSIONS: CD10 is a very useful immunomarker for distinguishing between G1-EEC and the metaplastic changes of EGBD in LBC samples | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a CD10 and CD31 | |
| 650 | 4 | |a endometrial liquid-based cytology | |
| 650 | 4 | |a fine fibrovascular stroma | |
| 650 | 4 | |a grade 1-endometrial endometrioid carcinoma | |
| 650 | 4 | |a immunocytochemistry | |
| 650 | 7 | |a CD146 Antigen |2 NLM | |
| 650 | 7 | |a PECAM1 protein, human |2 NLM | |
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| 700 | 1 | |a Nishikawa, Takeshi |e verfasserin |4 aut | |
| 700 | 1 | |a Suzuki, Hisae |e verfasserin |4 aut | |
| 700 | 1 | |a Hosokawa, Sho |e verfasserin |4 aut | |
| 700 | 1 | |a Yano, Hiroko |e verfasserin |4 aut | |
| 700 | 1 | |a Maeda, Yoshinobu |e verfasserin |4 aut | |
| 700 | 1 | |a Kurokawa, Tetsuji |e verfasserin |4 aut | |
| 700 | 1 | |a Shinagawa, Akiko |e verfasserin |4 aut | |
| 700 | 1 | |a Kobayashi, Tadao K |e verfasserin |4 aut | |
| 700 | 1 | |a Fulciniti, Franco |e verfasserin |4 aut | |
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