Feasibility and Diagnostic Accuracy of Saliva-Based SARS-CoV-2 Screening in Educational Settings and Children Aged <12 Years
Children have been disproportionately affected during the COVID-19 pandemic. We aimed to assess a saliva-based algorithm for SARS-CoV-2 testing to be used in schools and childcare institutions under pandemic conditions. A weekly SARS-CoV-2 sentinel study in primary schools, kindergartens, and childcare facilities was conducted over a 12-week-period. In a sub-study covering 7 weeks, 1895 paired oropharyngeal and saliva samples were processed for SARS-CoV-2 rRT-PCR testing in both asymptomatic children (n = 1243) and staff (n = 652). Forty-nine additional concurrent swab and saliva samples were collected from SARS-CoV-2 infected patients (patient cohort). The Salivette® system was used for saliva collection and assessed for feasibility and diagnostic performance. For children, a mean of 1.18 mL saliva could be obtained. Based on results from both cohorts, the Salivette® testing algorithm demonstrated the specificity of 100% (95% CI 99.7-100) and sensitivity of 94.9% (95% CI 81.4-99.1) with oropharyngeal swabs as reference. Agreement between sampling systems was 100% for moderate to high viral load situations (defined as Ct-values <33 from oropharyngeal swabs). Comparative analysis of Ct-values derived from saliva vs. oropharyngeal swabs demonstrated a significant difference (mean 4.23; 95% CI 2.48-6.00). In conclusion, the Salivette® system proved to be an easy-to-use, safe and feasible saliva collection method and a more pleasant alternative to oropharyngeal swabs for SARS-CoV-2 testing in children aged 3 years and above.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:11 |
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| Enthalten in: |
Diagnostics (Basel, Switzerland) - 11(2021), 10 vom: 29. Sept. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Hoch, Martin [VerfasserIn] |
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| Links: |
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| Themen: |
Childcare facilities |
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| Anmerkungen: |
Date Revised 26.10.2021 published: Electronic Citation Status PubMed-not-MEDLINE |
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| doi: |
10.3390/diagnostics11101797 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM332196364 |
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| 520 | |a Children have been disproportionately affected during the COVID-19 pandemic. We aimed to assess a saliva-based algorithm for SARS-CoV-2 testing to be used in schools and childcare institutions under pandemic conditions. A weekly SARS-CoV-2 sentinel study in primary schools, kindergartens, and childcare facilities was conducted over a 12-week-period. In a sub-study covering 7 weeks, 1895 paired oropharyngeal and saliva samples were processed for SARS-CoV-2 rRT-PCR testing in both asymptomatic children (n = 1243) and staff (n = 652). Forty-nine additional concurrent swab and saliva samples were collected from SARS-CoV-2 infected patients (patient cohort). The Salivette® system was used for saliva collection and assessed for feasibility and diagnostic performance. For children, a mean of 1.18 mL saliva could be obtained. Based on results from both cohorts, the Salivette® testing algorithm demonstrated the specificity of 100% (95% CI 99.7-100) and sensitivity of 94.9% (95% CI 81.4-99.1) with oropharyngeal swabs as reference. Agreement between sampling systems was 100% for moderate to high viral load situations (defined as Ct-values <33 from oropharyngeal swabs). Comparative analysis of Ct-values derived from saliva vs. oropharyngeal swabs demonstrated a significant difference (mean 4.23; 95% CI 2.48-6.00). In conclusion, the Salivette® system proved to be an easy-to-use, safe and feasible saliva collection method and a more pleasant alternative to oropharyngeal swabs for SARS-CoV-2 testing in children aged 3 years and above | ||
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| 700 | 1 | |a Eberle, Ute |e verfasserin |4 aut | |
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| 700 | 1 | |a Gruenthaler, Valerie |e verfasserin |4 aut | |
| 700 | 1 | |a Fingerle, Volker |e verfasserin |4 aut | |
| 700 | 1 | |a Ackermann, Nikolaus |e verfasserin |4 aut | |
| 700 | 1 | |a Sing, Andreas |e verfasserin |4 aut | |
| 700 | 1 | |a Liebl, Bernhard |e verfasserin |4 aut | |
| 700 | 1 | |a Huebner, Johannes |e verfasserin |4 aut | |
| 700 | 1 | |a Kuttiadan, Simone |e verfasserin |4 aut | |
| 700 | 1 | |a Rack-Hoch, Anita |e verfasserin |4 aut | |
| 700 | 1 | |a Meyer-Buehn, Melanie |e verfasserin |4 aut | |
| 700 | 1 | |a Schober, Tilmann |e verfasserin |4 aut | |
| 700 | 1 | |a von Both, Ulrich |e verfasserin |4 aut | |
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