SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart
© 2021 Valkov et al..
Extracellular vesicles (EVs) mediate intercellular signaling by transferring their cargo to recipient cells, but the functional consequences of signaling are not fully appreciated. RBC-derived EVs are abundant in circulation and have been implicated in regulating immune responses. Here, we use a transgenic mouse model for fluorescence-based mapping of RBC-EV recipient cells to assess the role of this intercellular signaling mechanism in heart disease. Using fluorescent-based mapping, we detected an increase in RBC-EV-targeted cardiomyocytes in a murine model of ischemic heart failure. Single cell nuclear RNA sequencing of the heart revealed a complex landscape of cardiac cells targeted by RBC-EVs, with enrichment of genes implicated in cell proliferation and stress signaling pathways compared with non-targeted cells. Correspondingly, cardiomyocytes targeted by RBC-EVs more frequently express cellular markers of DNA synthesis, suggesting the functional significance of EV-mediated signaling. In conclusion, our mouse model for mapping of EV-recipient cells reveals a complex cellular network of RBC-EV-mediated intercellular communication in ischemic heart failure and suggests a functional role for this mode of intercellular signaling.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:4 |
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| Enthalten in: |
Life science alliance - 4(2021), 12 vom: 01. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Valkov, Nedyalka [VerfasserIn] |
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| Links: |
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| Themen: |
Journal Article |
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| Anmerkungen: |
Date Completed 22.03.2022 Date Revised 17.06.2023 published: Electronic-Print Dryad: 10.5061/dryad.vhhmgqnv9 Citation Status MEDLINE |
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| doi: |
10.26508/lsa.202101048 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| 245 | 1 | 0 | |a SnRNA sequencing defines signaling by RBC-derived extracellular vesicles in the murine heart |
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| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 Valkov et al. | ||
| 520 | |a Extracellular vesicles (EVs) mediate intercellular signaling by transferring their cargo to recipient cells, but the functional consequences of signaling are not fully appreciated. RBC-derived EVs are abundant in circulation and have been implicated in regulating immune responses. Here, we use a transgenic mouse model for fluorescence-based mapping of RBC-EV recipient cells to assess the role of this intercellular signaling mechanism in heart disease. Using fluorescent-based mapping, we detected an increase in RBC-EV-targeted cardiomyocytes in a murine model of ischemic heart failure. Single cell nuclear RNA sequencing of the heart revealed a complex landscape of cardiac cells targeted by RBC-EVs, with enrichment of genes implicated in cell proliferation and stress signaling pathways compared with non-targeted cells. Correspondingly, cardiomyocytes targeted by RBC-EVs more frequently express cellular markers of DNA synthesis, suggesting the functional significance of EV-mediated signaling. In conclusion, our mouse model for mapping of EV-recipient cells reveals a complex cellular network of RBC-EV-mediated intercellular communication in ischemic heart failure and suggests a functional role for this mode of intercellular signaling | ||
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| 700 | 1 | |a Tucker, Nathan R |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Guoping |e verfasserin |4 aut | |
| 700 | 1 | |a Salvador, Ane M |e verfasserin |4 aut | |
| 700 | 1 | |a Chaffin, Mark D |e verfasserin |4 aut | |
| 700 | 1 | |a Pereira De Oliveira Junior, Getulio |e verfasserin |4 aut | |
| 700 | 1 | |a Kur, Ivan |e verfasserin |4 aut | |
| 700 | 1 | |a Gokulnath, Priyanka |e verfasserin |4 aut | |
| 700 | 1 | |a Ziegler, Olivia |e verfasserin |4 aut | |
| 700 | 1 | |a Yeri, Ashish |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Shulin |e verfasserin |4 aut | |
| 700 | 1 | |a Khamesra, Aushee |e verfasserin |4 aut | |
| 700 | 1 | |a Xiao, Chunyang |e verfasserin |4 aut | |
| 700 | 1 | |a Rodosthenous, Rodosthenis |e verfasserin |4 aut | |
| 700 | 1 | |a Srinivasan, Srimeenakshi |e verfasserin |4 aut | |
| 700 | 1 | |a Toxavidis, Vasilis |e verfasserin |4 aut | |
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