Notch Intracellular Domain Plasmid Delivery via Poly(Lactic-Co-Glycolic Acid) Nanoparticles to Upregulate Notch Pathway Molecules
Copyright © 2021 Messerschmidt, Chintapula, Kuriakose, Laboy, Truong, Kydd, Jaworski, Pan, Sadek, Nguyen and Lee..
Notch signaling is a highly conserved signaling system that is required for embryonic development and regeneration of organs. When the signal is lost, maldevelopment occurs and leads to a lethal state. Delivering exogenous genetic materials encoding Notch into cells can reestablish downstream signaling and rescue cellular functions. In this study, we utilized the negatively charged and FDA approved polymer poly(lactic-co-glycolic acid) to encapsulate Notch Intracellular Domain-containing plasmid in nanoparticles. We show that primary human umbilical vein endothelial cells (HUVECs) readily uptake the nanoparticles with and without specific antibody targets. We demonstrated that our nanoparticles are non-toxic, stable over time, and compatible with blood. We further demonstrated that HUVECs could be successfully transfected with these nanoparticles in static and dynamic environments. Lastly, we elucidated that these nanoparticles could upregulate the downstream genes of Notch signaling, indicating that the payload was viable and successfully altered the genetic downstream effects.
| Errataetall: |
ErratumIn: Front Cardiovasc Med. 2021 Oct 26;8:785910. - PMID 34778422 |
|---|---|
| Medienart: |
E-Artikel |
| Erscheinungsjahr: |
2021 |
|---|---|
| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:8 |
|---|---|
| Enthalten in: |
Frontiers in cardiovascular medicine - 8(2021) vom: 15., Seite 707897 |
| Sprache: |
Englisch |
|---|
| Beteiligte Personen: |
Messerschmidt, Victoria L [VerfasserIn] |
|---|
| Links: |
|---|
| Themen: |
Gene delivery |
|---|
| Anmerkungen: |
Date Revised 15.11.2021 published: Electronic-eCollection ErratumIn: Front Cardiovasc Med. 2021 Oct 26;8:785910. - PMID 34778422 Citation Status PubMed-not-MEDLINE |
|---|
| doi: |
10.3389/fcvm.2021.707897 |
|---|
| funding: |
|
|---|---|
| Förderinstitution / Projekttitel: |
|
| PPN (Katalog-ID): |
NLM331917440 |
|---|
| LEADER | 01000naa a22002652 4500 | ||
|---|---|---|---|
| 001 | NLM331917440 | ||
| 003 | DE-627 | ||
| 005 | 20231225214528.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 231225s2021 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.3389/fcvm.2021.707897 |2 doi | |
| 028 | 5 | 2 | |a pubmed24n1106.xml |
| 035 | |a (DE-627)NLM331917440 | ||
| 035 | |a (NLM)34651022 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 100 | 1 | |a Messerschmidt, Victoria L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Notch Intracellular Domain Plasmid Delivery via Poly(Lactic-Co-Glycolic Acid) Nanoparticles to Upregulate Notch Pathway Molecules |
| 264 | 1 | |c 2021 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ƒaComputermedien |b c |2 rdamedia | ||
| 338 | |a ƒa Online-Ressource |b cr |2 rdacarrier | ||
| 500 | |a Date Revised 15.11.2021 | ||
| 500 | |a published: Electronic-eCollection | ||
| 500 | |a ErratumIn: Front Cardiovasc Med. 2021 Oct 26;8:785910. - PMID 34778422 | ||
| 500 | |a Citation Status PubMed-not-MEDLINE | ||
| 520 | |a Copyright © 2021 Messerschmidt, Chintapula, Kuriakose, Laboy, Truong, Kydd, Jaworski, Pan, Sadek, Nguyen and Lee. | ||
| 520 | |a Notch signaling is a highly conserved signaling system that is required for embryonic development and regeneration of organs. When the signal is lost, maldevelopment occurs and leads to a lethal state. Delivering exogenous genetic materials encoding Notch into cells can reestablish downstream signaling and rescue cellular functions. In this study, we utilized the negatively charged and FDA approved polymer poly(lactic-co-glycolic acid) to encapsulate Notch Intracellular Domain-containing plasmid in nanoparticles. We show that primary human umbilical vein endothelial cells (HUVECs) readily uptake the nanoparticles with and without specific antibody targets. We demonstrated that our nanoparticles are non-toxic, stable over time, and compatible with blood. We further demonstrated that HUVECs could be successfully transfected with these nanoparticles in static and dynamic environments. Lastly, we elucidated that these nanoparticles could upregulate the downstream genes of Notch signaling, indicating that the payload was viable and successfully altered the genetic downstream effects | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Notch signaling | |
| 650 | 4 | |a PLGA | |
| 650 | 4 | |a gene delivery | |
| 650 | 4 | |a nanoparticles | |
| 650 | 4 | |a non-viral transfection | |
| 700 | 1 | |a Chintapula, Uday |e verfasserin |4 aut | |
| 700 | 1 | |a Kuriakose, Aneetta E |e verfasserin |4 aut | |
| 700 | 1 | |a Laboy, Samantha |e verfasserin |4 aut | |
| 700 | 1 | |a Truong, Thuy Thi Dang |e verfasserin |4 aut | |
| 700 | 1 | |a Kydd, LeNaiya A |e verfasserin |4 aut | |
| 700 | 1 | |a Jaworski, Justyn |e verfasserin |4 aut | |
| 700 | 1 | |a Pan, Zui |e verfasserin |4 aut | |
| 700 | 1 | |a Sadek, Hashem |e verfasserin |4 aut | |
| 700 | 1 | |a Nguyen, Kytai T |e verfasserin |4 aut | |
| 700 | 1 | |a Lee, Juhyun |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Frontiers in cardiovascular medicine |d 2014 |g 8(2021) vom: 15., Seite 707897 |w (DE-627)NLM255534736 |x 2297-055X |7 nnns |
| 773 | 1 | 8 | |g volume:8 |g year:2021 |g day:15 |g pages:707897 |
| 856 | 4 | 0 | |u http://dx.doi.org/10.3389/fcvm.2021.707897 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a GBV_NLM | ||
| 951 | |a AR | ||
| 952 | |d 8 |j 2021 |b 15 |h 707897 | ||