Mitochondrial phylogenetic and diversity analysis in Azi-Kheli buffalo
© 2021. The Author(s), under exclusive licence to Springer Nature B.V..
NOVELTY STATEMENT: The present study was conducted for the first time in Pakistan to investigate Cytochrome C Oxidase Subunit 1 (CO1) gene and full-length Displacement Loop (D-loop) region of mitochondrial DNA in Azi-Kheli buffalo breed native to northern hilly areas of Khyber Pakhtunkhwa Province of Pakistan. The present study was designed to investigate phylogeny and diversity in Azi-Kheli buffalo, through two mitochondrial DNA regions, i.e., Cytochrome C Oxidase Subunit-I (CO1) and Displacement Loop (D-loop) region. Thirty (30) blood samples were taken from Azi-Kheli pure breed animals from original breeding tract, i.e., Khwazakhela, Swat. Polymerase chain reactions using gene-specific primers were carried out for amplifying 709-bp region of CO1 gene and 1159-bp region of D-Loop for identification, phylogeny, and diversity in Azi-Kheli buffalo, respectively. The sequences of CO1 gene revealed four (04) haplotypes, whereas D-loop sequences revealed five (05) haplotypes. Mean interspecific diversity with related species was 2.56%, and mean intraspecific diversity within Azi-Kheli buffalo was 0.25%, estimated via Kimura-2 parameter. Phylogenetic tree (maximum likelihood) revealed clustering of Azi-Kheli haplotypes with river buffalo and is distinct from swamp buffalo and other related species of genus Bubalus. Mean haplotype and nucleotide diversity of D-loop were Hd = 0.9601 ± SD = 0.096 and π = 0.01208 ± SD = 0.00182, respectively. Phylogenetic tree (neighbor-joining) revealed two main clades, i.e., river buffalo and swamp buffalo clade. The haplotypes of Azi-Kheli clustered with haplotypes of different river buffalo breeds at different positions. The current study suggests that Azi-Kheli has common origin with other river buffalo breeds; hence, it is river buffalo which harbors high genetic diversity.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:53 |
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| Enthalten in: |
Tropical animal health and production - 53(2021), 5 vom: 12. Okt., Seite 512 |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Ali, Farhad [VerfasserIn] |
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| Links: |
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| Themen: |
Azi-Kheli buffalo |
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| Anmerkungen: |
Date Completed 14.10.2021 Date Revised 14.10.2021 published: Electronic Citation Status MEDLINE |
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| doi: |
10.1007/s11250-021-02949-z |
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| funding: |
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| Förderinstitution / Projekttitel: |
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NLM33177870X |
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| 245 | 1 | 0 | |a Mitochondrial phylogenetic and diversity analysis in Azi-Kheli buffalo |
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| 520 | |a © 2021. The Author(s), under exclusive licence to Springer Nature B.V. | ||
| 520 | |a NOVELTY STATEMENT: The present study was conducted for the first time in Pakistan to investigate Cytochrome C Oxidase Subunit 1 (CO1) gene and full-length Displacement Loop (D-loop) region of mitochondrial DNA in Azi-Kheli buffalo breed native to northern hilly areas of Khyber Pakhtunkhwa Province of Pakistan. The present study was designed to investigate phylogeny and diversity in Azi-Kheli buffalo, through two mitochondrial DNA regions, i.e., Cytochrome C Oxidase Subunit-I (CO1) and Displacement Loop (D-loop) region. Thirty (30) blood samples were taken from Azi-Kheli pure breed animals from original breeding tract, i.e., Khwazakhela, Swat. Polymerase chain reactions using gene-specific primers were carried out for amplifying 709-bp region of CO1 gene and 1159-bp region of D-Loop for identification, phylogeny, and diversity in Azi-Kheli buffalo, respectively. The sequences of CO1 gene revealed four (04) haplotypes, whereas D-loop sequences revealed five (05) haplotypes. Mean interspecific diversity with related species was 2.56%, and mean intraspecific diversity within Azi-Kheli buffalo was 0.25%, estimated via Kimura-2 parameter. Phylogenetic tree (maximum likelihood) revealed clustering of Azi-Kheli haplotypes with river buffalo and is distinct from swamp buffalo and other related species of genus Bubalus. Mean haplotype and nucleotide diversity of D-loop were Hd = 0.9601 ± SD = 0.096 and π = 0.01208 ± SD = 0.00182, respectively. Phylogenetic tree (neighbor-joining) revealed two main clades, i.e., river buffalo and swamp buffalo clade. The haplotypes of Azi-Kheli clustered with haplotypes of different river buffalo breeds at different positions. The current study suggests that Azi-Kheli has common origin with other river buffalo breeds; hence, it is river buffalo which harbors high genetic diversity | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Azi-Kheli buffalo | |
| 650 | 4 | |a CO1 | |
| 650 | 4 | |a D-loop | |
| 650 | 4 | |a Genetic diversity | |
| 650 | 4 | |a Mitochondrial DNA | |
| 650 | 4 | |a Phylogenetics | |
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| 700 | 1 | |a Ali, Muhammad Ijaz |e verfasserin |4 aut | |
| 700 | 1 | |a Riaz, Muhammad Hasnain |e verfasserin |4 aut | |
| 700 | 1 | |a Khan, Nadar |e verfasserin |4 aut | |
| 700 | 1 | |a Ullah, Obaid |e verfasserin |4 aut | |
| 700 | 1 | |a Hassan, Faizul |e verfasserin |4 aut | |
| 700 | 1 | |a Suhail, Syed Muhammad |e verfasserin |4 aut | |
| 700 | 1 | |a Khan, Rajwali |e verfasserin |4 aut | |
| 700 | 1 | |a Khan, Muhammad Tahir |e verfasserin |4 aut | |
| 700 | 1 | |a Ahmad, Irshad |e verfasserin |4 aut | |
| 700 | 1 | |a Zeb, Muhammad Tariq |e verfasserin |4 aut | |
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