Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle
© 2021 Lo et al..
The cavin proteins are essential for caveola biogenesis and function. Here, we identify a role for the muscle-specific component, Cavin4, in skeletal muscle T-tubule development by analyzing two vertebrate systems, mouse and zebrafish. In both models, Cavin4 localized to T-tubules, and loss of Cavin4 resulted in aberrant T-tubule maturation. In zebrafish, which possess duplicated cavin4 paralogs, Cavin4b was shown to directly interact with the T-tubule-associated BAR domain protein Bin1. Loss of both Cavin4a and Cavin4b caused aberrant accumulation of interconnected caveolae within the T-tubules, a fragmented T-tubule network enriched in Caveolin-3, and an impaired Ca2+ response upon mechanical stimulation. We propose a role for Cavin4 in remodeling the T-tubule membrane early in development by recycling caveolar components from the T-tubule to the sarcolemma. This generates a stable T-tubule domain lacking caveolae that is essential for T-tubule function.
| Medienart: |
E-Artikel |
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| Erscheinungsjahr: |
2021 |
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| Erschienen: |
2021 |
| Enthalten in: |
Zur Gesamtaufnahme - volume:220 |
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| Enthalten in: |
The Journal of cell biology - 220(2021), 12 vom: 06. Dez. |
| Sprache: |
Englisch |
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| Beteiligte Personen: |
Lo, Harriet P [VerfasserIn] |
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| Links: |
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| Anmerkungen: |
Date Completed 22.12.2021 Date Revised 26.02.2024 published: Print-Electronic Citation Status MEDLINE |
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| doi: |
10.1083/jcb.201905065 |
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| funding: |
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| Förderinstitution / Projekttitel: |
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| PPN (Katalog-ID): |
NLM331743221 |
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| 100 | 1 | |a Lo, Harriet P |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle |
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| 500 | |a Date Completed 22.12.2021 | ||
| 500 | |a Date Revised 26.02.2024 | ||
| 500 | |a published: Print-Electronic | ||
| 500 | |a Citation Status MEDLINE | ||
| 520 | |a © 2021 Lo et al. | ||
| 520 | |a The cavin proteins are essential for caveola biogenesis and function. Here, we identify a role for the muscle-specific component, Cavin4, in skeletal muscle T-tubule development by analyzing two vertebrate systems, mouse and zebrafish. In both models, Cavin4 localized to T-tubules, and loss of Cavin4 resulted in aberrant T-tubule maturation. In zebrafish, which possess duplicated cavin4 paralogs, Cavin4b was shown to directly interact with the T-tubule-associated BAR domain protein Bin1. Loss of both Cavin4a and Cavin4b caused aberrant accumulation of interconnected caveolae within the T-tubules, a fragmented T-tubule network enriched in Caveolin-3, and an impaired Ca2+ response upon mechanical stimulation. We propose a role for Cavin4 in remodeling the T-tubule membrane early in development by recycling caveolar components from the T-tubule to the sarcolemma. This generates a stable T-tubule domain lacking caveolae that is essential for T-tubule function | ||
| 650 | 4 | |a Journal Article | |
| 650 | 4 | |a Research Support, Non-U.S. Gov't | |
| 650 | 7 | |a Adaptor Proteins, Signal Transducing |2 NLM | |
| 650 | 7 | |a Bin1 protein, mouse |2 NLM | |
| 650 | 7 | |a Cavin4 protein, mouse |2 NLM | |
| 650 | 7 | |a Membrane Proteins |2 NLM | |
| 650 | 7 | |a Muscle Proteins |2 NLM | |
| 650 | 7 | |a Nerve Tissue Proteins |2 NLM | |
| 650 | 7 | |a Tumor Suppressor Proteins |2 NLM | |
| 650 | 7 | |a Zebrafish Proteins |2 NLM | |
| 650 | 7 | |a bin1b protein, zebrafish |2 NLM | |
| 650 | 7 | |a cavin4b protein, zebrafish |2 NLM | |
| 700 | 1 | |a Lim, Ye-Wheen |e verfasserin |4 aut | |
| 700 | 1 | |a Xiong, Zherui |e verfasserin |4 aut | |
| 700 | 1 | |a Martel, Nick |e verfasserin |4 aut | |
| 700 | 1 | |a Ferguson, Charles |e verfasserin |4 aut | |
| 700 | 1 | |a Ariotti, Nicholas |e verfasserin |4 aut | |
| 700 | 1 | |a Giacomotto, Jean |e verfasserin |4 aut | |
| 700 | 1 | |a Rae, James |e verfasserin |4 aut | |
| 700 | 1 | |a Floetenmeyer, Matthias |e verfasserin |4 aut | |
| 700 | 1 | |a Moradi, Shayli Varasteh |e verfasserin |4 aut | |
| 700 | 1 | |a Gao, Ya |e verfasserin |4 aut | |
| 700 | 1 | |a Tillu, Vikas A |e verfasserin |4 aut | |
| 700 | 1 | |a Xia, Di |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Huang |e verfasserin |4 aut | |
| 700 | 1 | |a Rahnama, Samira |e verfasserin |4 aut | |
| 700 | 1 | |a Nixon, Susan J |e verfasserin |4 aut | |
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| 700 | 1 | |a Parton, Robert G |e verfasserin |4 aut | |
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